Description
Antibody Name: | Anti-MAN2A2 Antibody (CAB19323) |
Antibody SKU: | CAB19323 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human |
Host Species: | Rabbit |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MAN2A2 (NP_006113.2). |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human |
Positive Samples: | U-87MG |
Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 100-200 of human MAN2A2 (NP_006113.2). |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | VPPE PRPS FFSI SPQD CQFA LGGR GQKP ELQM LTVS EELP FDNV DGGV WRQG FDIS YDPH DWDA EDLQ VFVV PHSH NDPG WIKT FDKY YTEQ TQHI LNSM V |
Cellular Location: | |
Calculated MW: | |
Observed MW: | 128KDa |
Synonyms: | MANA2X, MAN2A2 |
Background: | Catalyzes the first committed step in the biosynthesis of complex N-glycans. It controls conversion of high mannose to complex N-glycans; the final hydrolytic step in the N-glycan maturation pathway. |
Western blot analysis of extracts of U-87MG cells, using MAN2A2 antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s. |