Cell Biology Antibodies 17

Anti-macroH2A.1 Antibody (CAB9059)

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SKU:
CAB9059
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Monoclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-macroH2A.1 Antibody
Antibody SKU:CAB9059
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthesized peptide derived from human macroH2A.1
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, 293T, HepG2
Immunogen:A synthesized peptide derived from human macroH2A.1
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:9555
Uniprot:O75367
Cellular Location:
Calculated MW:40kDa
Observed MW:41KDa
Synonyms:H2A.y, H2A/y, H2AF12M, MACROH2A1.1, mH2A1, macroH2A1.2
Background:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a replication-independent histone that is a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2015]
UniProt Protein Function:H2AFY: Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post- translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces an hypoacetylated state of chromatin. In addition, isoform 1, but not isoform 2, binds ADP-ribose and O-acetyl-ADP- ribose, and may be involved in ADP-ribose-mediated chromatin modulation. The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. Interacts with SPOP. Part of a complex consisting of H2AFY, CUL3 and SPOP. Interacts with HDAC1 and HDAC2. Ubiquitous. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding

Chromosomal Location of Human Ortholog: 5q31.1

Cellular Component: nucleoplasm; centric heterochromatin; sex chromatin; ESC/E(Z) complex; nuclear chromatin; nucleolus; Barr body; condensed chromosome; nucleosome; nucleus

Molecular Function:rDNA binding; protein serine/threonine kinase inhibitor activity; protein binding; DNA binding; double-stranded methylated DNA binding; chromatin DNA binding; protein heterodimerization activity; protein kinase binding

Biological Process: nucleosome assembly; negative regulation of gene expression, epigenetic; dosage compensation; negative regulation of transcription from RNA polymerase II promoter; chromatin modification; regulation of cell growth; negative regulation of histone phosphorylation

NCBI Summary:Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Nucleosomes consist of approximately 146 bp of DNA wrapped around a histone octamer composed of pairs of each of the four core histones (H2A, H2B, H3, and H4). The chromatin fiber is further compacted through the interaction of a linker histone, H1, with the DNA between the nucleosomes to form higher order chromatin structures. This gene encodes a member of the histone H2A family. It replaces conventional H2A histones in a subset of nucleosomes where it represses transcription and participates in stable X chromosome inactivation. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:O75367
NCBI GenInfo Identifier:90110023
NCBI Gene ID:9555
NCBI Accession:O75367.4
UniProt Secondary Accession:O75367,O75377, Q503A8, Q7Z5E3, Q96D41, Q9H8P3, Q9UP96
UniProt Related Accession:O75367
Molecular Weight:372
NCBI Full Name:Core histone macro-H2A.1
NCBI Synonym Full Names:H2A histone family, member Y
NCBI Official Symbol:H2AFY  
NCBI Official Synonym Symbols:H2A.y; H2A/y; H2AFJ; mH2A1; H2AF12M; MACROH2A1.1; macroH2A1.2  
NCBI Protein Information:core histone macro-H2A.1; histone H2A.y; histone macroH2A1; histone macroH2A1.1; histone macroH2A1.2; medulloblastoma antigen MU-MB-50.205
UniProt Protein Name:Core histone macro-H2A.1
UniProt Synonym Protein Names:Histone H2A.y; H2A/y; Medulloblastoma antigen MU-MB-50.205
Protein Family:Core histone macro
UniProt Gene Name:H2AFY  
UniProt Entry Name:H2AY_HUMAN
Anti-macroH2A.1 Antibody (CAB9059)Western blot - macroH2A.1 Rabbit mAb (CAB9059)
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