Immunology Antibodies 2
Anti-LY96 Antibody (CAB1866)
- SKU:
- CAB1866
- Product Type:
- Antibody
- Reactivity:
- Mouse
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-LY96 Antibody |
| Antibody SKU: | CAB1866 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Mouse |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human LY96 (NP_056179.3). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Mouse |
| Positive Samples: | Mouse liver, Mouse spleen, Mouse kidney |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 19-160 of human LY96 (NP_056179.3). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | QKQY WVCN SSDA SISY TYCD KMQY PISI NVNP CIEL KGSK GLLH IFYI PRRD LKQL YFNL YITV NTMN LPKR KEVI CRGS DDDY SFCR ALKG ETVN TTIS FSFK GIKF SKGK YKCV VEAI SGSP EEML FCLE FVIL HQPN SN |
| Gene ID: | 23643 |
| Uniprot: | Q9Y6Y9 |
| Cellular Location: | Secreted, extracellular space |
| Calculated MW: | 14kDa/18kDa |
| Observed MW: | 25kDa |
| Synonyms: | LY96, ESOP-1, MD-2, MD2, ly-96 |
| Background: | This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms. |
| UniProt Protein Function: | LY96: a secreted protein that binds to the extracellular domains of TLR2 and TLR4. Cooperates with TLR4 in the innate immune response to bacterial lipopolysaccharide (LPS), and with TLR2 in the response to cell wall components from Gram-positive and Gram-negative bacteria. Enhances TLR4-dependent activation of NF-kappa-B. Cells expressing both LY96 and TLR4, but not TLR4 alone, respond to LPS. Heterogeneous homopolymer formed from homodimers; disulfide-linked. Belongs to the lipopolysaccharide (LPS) receptor complex, a multi-protein complex containing at least CD14, LY96 and TLR4. Ligand binding induces interaction with TLR4 and oligomerization of the complex. |
| UniProt Protein Details: | Protein type:Secreted, signal peptide; Secreted Chromosomal Location of Human Ortholog: 8q21.11 Cellular Component: extracellular space; plasma membrane; lipopolysaccharide receptor complex; endosome membrane Molecular Function:protein binding; lipopolysaccharide receptor activity; coreceptor activity Biological Process: I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; positive regulation of lipopolysaccharide-mediated signaling pathway; response to lipopolysaccharide; toll-like receptor 3 signaling pathway; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; cell surface receptor linked signal transduction; detection of lipopolysaccharide; lipopolysaccharide-mediated signaling pathway; toll-like receptor signaling pathway; innate immune response; cellular defense response; inflammatory response; toll-like receptor 4 signaling pathway |
| NCBI Summary: | This gene encodes a protein which associates with toll-like receptor 4 on the cell surface and confers responsiveness to lipopolysaccyaride (LPS), thus providing a link between the receptor and LPS signaling. Studies of the mouse ortholog suggest that this gene may be involved in endotoxin neutralization. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Sep 2010] |
| UniProt Code: | Q9Y6Y9 |
| NCBI GenInfo Identifier: | 296434574 |
| NCBI Gene ID: | 23643 |
| NCBI Accession: | Q9Y6Y9.2 |
| UniProt Secondary Accession: | Q9Y6Y9,B3Y6A5, E5RJJ7, |
| UniProt Related Accession: | Q9Y6Y9 |
| Molecular Weight: | 160 |
| NCBI Full Name: | Lymphocyte antigen 96 |
| NCBI Synonym Full Names: | lymphocyte antigen 96 |
| NCBI Official Symbol: | LY96 |
| NCBI Official Synonym Symbols: | MD2; MD-2; ly-96; ESOP-1 |
| NCBI Protein Information: | lymphocyte antigen 96; protein MD-2; myeloid differentiation protein-2 |
| UniProt Protein Name: | Lymphocyte antigen 96 |
| UniProt Synonym Protein Names: | ESOP-1; Protein MD-2 |
| Protein Family: | Lymphocyte antigen |
| UniProt Gene Name: | LY96 |
| UniProt Entry Name: | LY96_HUMAN |
 | Western blot analysis of extracts of various cell lines, using LY96 antibody (CAB1866) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
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