KO Validated Antibodies 2

Anti-LTA4H Antibody (CAB19940)[KO Validated]

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SKU:
CAB19940
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Metabolism

Description

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Antibody Name:Anti-LTA4H Antibody [KO Validated]
Antibody SKU:CAB19940
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant protein of human LTA4H.
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse
Positive Samples:293T
Immunogen:Recombinant protein of human LTA4H.
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:Email for sequence
Gene ID:4048
Uniprot:P09960
Cellular Location:Cytoplasm
Calculated MW:57kDa/59kDa/66kDa/69kDa
Observed MW:75KDa
Anti-LTA4H Antibody [KO Validated] (CAB19940)Western blot analysis of extracts from normal (control) and LTA4H knockout (KO) 293T cells, using LTA4H antibody (CAB19940) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Synonyms:LTA4H
Background:The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:LTA4H: Epoxide hydrolase that catalyzes the final step in the biosynthesis of the proinflammatory mediator leukotriene B4. Has also aminopeptidase activity. Belongs to the peptidase M1 family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:EC 3.3.2.6; Hydrolase; Lipid Metabolism - arachidonic acid

Chromosomal Location of Human Ortholog: 12q22

Cellular Component: nucleoplasm; cytoplasm; cytosol

Molecular Function:peptidase activity; leukotriene-A4 hydrolase activity; metallopeptidase activity; zinc ion binding; epoxide hydrolase activity; aminopeptidase activity

Biological Process: leukotriene biosynthetic process; peptide catabolic process; arachidonic acid metabolic process; leukotriene metabolic process; proteolysis; inflammatory response

NCBI Summary:The protein encoded by this gene is an enzyme that contains both hydrolase and aminopeptidase activities. The hydrolase activity is used in the final step of the biosynthesis of leukotriene B4, a proinflammatory mediator. The aminopeptidase activity has been shown to degrade proline-glycine-proline (PGP), a neutrophil chemoattractant and biomarker for chronic obstructive pulmonary disease (COPD). Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:P09960
NCBI GenInfo Identifier:126353
NCBI Gene ID:4048
NCBI Accession:P09960.2
UniProt Related Accession:P09960
Molecular Weight:~ 69kDa
NCBI Full Name:Leukotriene A-4 hydrolase
NCBI Synonym Full Names:leukotriene A4 hydrolase
NCBI Official Symbol:LTA4H  
NCBI Protein Information:leukotriene A-4 hydrolase
UniProt Protein Name:Leukotriene A-4 hydrolase
UniProt Synonym Protein Names:Leukotriene A(4) hydrolase
Protein Family:Leukotriene A-4 hydrolase
UniProt Gene Name:LTA4H  
UniProt Entry Name:LKHA4_HUMAN
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