Antibody Name:	Anti-LGR5 Antibody
Antibody SKU:	CAB10545
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5 (NP_003658.1).

Application:	WB IF
Recommended Dilution:	WB 1:1000 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HT-29, HepG2, SKOV3, A-431, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 824-907 of human LGR5 (NP_003658.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NPHF KEDL VSLR KQTY VWTR SKHP SLMS INSD DVEK QSCD STQA LVTF TSSS ITYD LPPS SVPS PAYP VTES CHLS SVAF VPCL
Gene ID:	8549
Uniprot:	O75473
Cellular Location:	Cell membrane, Golgi apparatus, Multi-pass membrane protein, trans-Golgi network membrane
Calculated MW:	92kDa/97kDa/99kDa
Observed MW:	105kDa

Synonyms:

LGR5, FEX, GPR49, GPR67, GRP49, HG38

Background:

The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	LGR5: a leucine-rich repeat-containing G-protein-coupled receptor. Receptor for R-spondins, potent stimulators of adult stem cell proliferation. A marker of cycling, long-lived and multipotent adult stem cell populations. Target gene of Wnt signaling. A Wnt receptor component that enhances Wnt signaling by soluble R-spondin proteins. Intestinal Lgr5+ stem cells give rise throughout life to proliferation of cells in crypts, which migrate along the crypt-villus axis, differentiating into five main cell types. Wnt signaling plays a crucial role in the proliferation and differentiation of these crypt epithelial cells. Two isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:GPCR, family 1; Membrane protein, integral; Membrane protein, multi-pass; Receptor, GPCR Chromosomal Location of Human Ortholog: 12q21.1 Cellular Component: integral to plasma membrane; plasma membrane; trans-Golgi network membrane Molecular Function:G-protein coupled receptor activity; protein binding; transmembrane receptor activity
NCBI Summary:	The protein encoded by this gene is a leucine-rich repeat-containing receptor (LGR) and member of the G protein-coupled, 7-transmembrane receptor (GPCR) superfamily. The encoded protein is a receptor for R-spondins and is involved in the canonical Wnt signaling pathway. This protein plays a role in the formation and maintenance of adult intestinal stem cells during postembryonic development. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2015]
UniProt Code:	O75473
NCBI GenInfo Identifier:	21542118
NCBI Gene ID:	8549
NCBI Accession:	O75473.1
UniProt Secondary Accession:	O75473,Q4VAM0, Q4VAM2, Q9UP75, D8MCT0,
UniProt Related Accession:	O75473
Molecular Weight:	100kDa
NCBI Full Name:	Leucine-rich repeat-containing G-protein coupled receptor 5
NCBI Synonym Full Names:	leucine rich repeat containing G protein-coupled receptor 5
NCBI Official Symbol:	LGR5
NCBI Official Synonym Symbols:	FEX; HG38; GPR49; GPR67; GRP49
NCBI Protein Information:	leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Protein Name:	Leucine-rich repeat-containing G-protein coupled receptor 5
UniProt Synonym Protein Names:	G-protein coupled receptor 49; G-protein coupled receptor 67; G-protein coupled receptor HG38
Protein Family:	Leucine-rich repeat-containing G-protein coupled receptor
UniProt Gene Name:	LGR5

	Western blot analysis of extracts of various cell lines, using LGR5 antibody (CAB10545) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of NIH/3T3 cells using LGR5 antibody (CAB10545) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of PC12 cells using LGR5 antibody (CAB10545) at dilution of 1:100.