Name: Anti-LGI1 Antibody (CAB5408)
Brand: Cell Biology Antibodies 9
SKU: CAB5408
Availability: OutOfStock

Antibody Name:	Anti-LGI1 Antibody
Antibody SKU:	CAB5408
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 301-557 of human LGI1 (NP_005088.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	U-87MG, HeLa, Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 301-557 of human LGI1 (NP_005088.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QLFG GSHI YKRD SFAN KFIK IQDI EILK IRKP NDIE TFKI ENNW YFVV ADSS KAGF TTIY KWNG NGFY SHQS LHAW YRDT DVEY LEIV RTPQ TLRT PHLI LSSS SQRP VIYQ WNKA TQLF TNQT DIPN MEDV YAVK HFSV KGDV YICL TRFI GDSK VMKW GGSS FQDI QRMP SRGS MVFQ PLQI NNYQ YAIL GSDY SFTQ VYNW DAEK AKFV KFQE LNVQ APRS FTHV SINK RNFL FASS FKGN TQIY KHVI VDLS A
Gene ID:	9211
Uniprot:	O95970
Cellular Location:	Cell junction, Secreted, synapse
Calculated MW:	33kDa/58kDa/63kDa
Observed MW:	64kDa

Synonyms:

LGI1, ADLTE, ADPAEF, ADPEAF, EPITEMPIN, EPT, ETL1, IB1099

Background:

This gene encodes a member of the secreted leucine-rich repeat (LRR) superfamily and shares homology with members of the SLIT protein family. The encoded protein may regulate the activity of voltage-gated potassium channels and may be involved in neuronal growth regulation and cell survival. This gene is rearranged as a result of translocations in glioblastoma cell lines, and it is frequently down-regulated or rearranged in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	LGI1: Regulates voltage-gated potassium channels assembled from KCNA1, KCNA4 and KCNAB1. It slows down channel inactivation by precluding channel closure mediated by the KCNAB1 subunit. Ligand for ADAM22 that positively regulates synaptic transmission mediated by AMPA-type glutamate receptors. Plays a role in suppressing the production of MMP1/3 through the phosphatidylinositol 3-kinase/ERK pathway. May play a role in the control of neuroblastoma cell survival. Defects in LGI1 are the cause of epilepsy, familial temporal lobe, type 1 (ETL1). A focal form of epilepsy characterized by recurrent seizures that arise from foci within the temporal lobe. Seizures are usually accompanied by sensory symptoms, most often auditory in nature. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Secreted; Secreted, signal peptide Chromosomal Location of Human Ortholog: 10q24 Cellular Component: extracellular space; extracellular region; synapse; cell junction Molecular Function:protein binding; receptor binding Biological Process: axon guidance; nervous system development; cell proliferation; positive regulation of cell growth; neurite development; protein homooligomerization; positive regulation of synaptic transmission Disease: Epilepsy, Familial Temporal Lobe, 1
NCBI Summary:	This gene is rearranged as a result of translocations in glioblastoma cell lines. The protein contains a hydrophobic segment representing a putative transmembrane domain with the amino terminus located outside the cell. It also contains leucine-rich repeats with conserved cysteine-rich flanking sequences. This gene is predominantly expressed in neural tissues and its expression is reduced in low grade brain tumors and significantly reduced or absent in malignant gliomas. Mutations in this gene result in autosomal dominant lateral temporal epilepsy. [provided by RefSeq, Jul 2008]
UniProt Code:	O95970
NCBI GenInfo Identifier:	32469669
NCBI Gene ID:	9211
NCBI Accession:	O95970.1
UniProt Secondary Accession:	O95970,Q5W001, Q5W002, Q8NI23, Q96LF5, A8K0Z1, B4E1S0
UniProt Related Accession:	O95970
Molecular Weight:	557
NCBI Full Name:	Leucine-rich glioma-inactivated protein 1
NCBI Synonym Full Names:	leucine-rich, glioma inactivated 1
NCBI Official Symbol:	LGI1
NCBI Official Synonym Symbols:	EPT; ETL1; ADLTE; ADPAEF; ADPEAF; IB1099; EPITEMPIN
NCBI Protein Information:	leucine-rich glioma-inactivated protein 1; epitempin-1
UniProt Protein Name:	Leucine-rich glioma-inactivated protein 1
UniProt Synonym Protein Names:	Epitempin-1
Protein Family:	Leucine-rich glioma-inactivated protein
UniProt Gene Name:	LGI1
UniProt Entry Name:	LGI1_HUMAN

Western blot analysis of extracts of various cell lines, using LGI1 antibody (CAB5408) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

LGI1 Antibody (PACO10240)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)