KO Validated Antibodies 1

Anti-LGALS1 Antibody (CAB18040)[KO Validated]

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SKU:
CAB18040
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Cell Death

Description

system_update_altDatasheetsystem_update_altMSDS
Antibody Name:Anti-LGALS1 Antibody [KO Validated]
Antibody SKU:CAB18040
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human LGALS1 (NP_002296.1).
Application:WB
Recommended Dilution:WB 1:1000 - 1:4000
Reactivity:Human, Mouse, Rat
Positive Samples:293T
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-135 of human LGALS1 (NP_002296.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MACG LVAS NLNL KPGE CLRV RGEV APDA KSFV LNLG KDSN NLCL HFNP RFNA HGDA NTIV CNSK DGGA WGTE QREA VFPF QPGS VAEV CITF DQAN LTVK LPDG YEFK FPNR LNLE AINY MAAD GDFK IKCV AFD
Gene ID:3956
Uniprot:P09382
Cellular Location:Secreted, extracellular matrix, extracellular space
Calculated MW:14kDa
Observed MW:14kDa
Anti-LGALS1 Antibody [KO Validated] (CAB18040)Western blot analysis of extracts from normal (control) and LGALS1 knockout (KO) 293T cells, using LGALS1 antibody (CAB18040) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 3min.
Synonyms:LGALS1, GAL1, GBP
Background:The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation.
UniProt Protein Function:Galectin-1: May regulate apoptosis, cell proliferation and cell differentiation. Binds beta-galactoside and a wide array of complex carbohydrates. Inhibits CD45 protein phosphatase activity and therefore the dephosphorylation of Lyn kinase. Homodimer. Binds LGALS3BP. Interacts with CD2, CD3, CD4, CD7, CD43 and CD45. Interacts with laminin (via poly-N- acetyllactosamine). Expressed in placenta, maternal decidua and fetal membranes. Within placenta, expressed in trophoblasts, stromal cells, villous endothelium, syncytiotrophoblast apical membrane and villous stroma. Within fetal membranes, expressed in amnion, chorioamniotic mesenchyma and chorion. Expressed in cardiac, smooth, and skeletal muscle, neurons, thymus, kidney and hematopoietic cells.
UniProt Protein Details:

Protein type:Cell adhesion

Chromosomal Location of Human Ortholog: 22q13.1

Cellular Component: extracellular matrix; extracellular space; proteinaceous extracellular matrix; cell surface; cytoplasm; intracellular; nucleus

Molecular Function:signal transducer activity; protein binding; protein homodimerization activity; lactose binding; galactoside binding; laminin binding; glycoprotein binding

Biological Process: regulation of apoptosis; response to drug; myoblast differentiation; positive regulation of I-kappaB kinase/NF-kappaB cascade; plasma cell differentiation; apoptosis; T cell costimulation; positive regulation of erythrocyte aggregation; response to axon injury; signal transduction; multicellular organismal response to stress

NCBI Summary:The galectins are a family of beta-galactoside-binding proteins implicated in modulating cell-cell and cell-matrix interactions. This gene product may act as an autocrine negative growth factor that regulates cell proliferation. [provided by RefSeq, Jul 2008]
UniProt Code:P09382
NCBI GenInfo Identifier:126155
NCBI Gene ID:3956
NCBI Accession:P09382.2
UniProt Secondary Accession:P09382,Q9UDK5, B2R5E8,
UniProt Related Accession:P09382
Molecular Weight:14,716 Da
NCBI Full Name:Galectin-1
NCBI Synonym Full Names:lectin, galactoside-binding, soluble, 1
NCBI Official Symbol:LGALS1  
NCBI Official Synonym Symbols:GBP; GAL1  
NCBI Protein Information:galectin-1; HBL; HPL; gal-1; HLBP14; galaptin; galectin 1; 14 kDa lectin; S-Lac lectin 1; lactose-binding lectin 1; 14 kDa laminin-binding protein; putative MAPK-activating protein PM12; beta-galactoside-binding lectin L-14-I; beta-galactoside-binding protein 14kDa
UniProt Protein Name:Galectin-1
UniProt Synonym Protein Names:14 kDa laminin-binding protein; HLBP14; 14 kDa lectin; Beta-galactoside-binding lectin L-14-I; Galaptin; HBL; HPL; Lactose-binding lectin 1; Lectin galactoside-binding soluble 1; Putative MAPK-activating protein PM12; S-Lac lectin 1
Protein Family:Galectin
UniProt Gene Name:LGALS1  
UniProt Entry Name:LEG1_HUMAN
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