Metabolism Antibodies 1

Anti-LDLR Antibody (CAB14996)

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SKU:
CAB14996
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Metabolism

Description

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Antibody Name:Anti-LDLR Antibody
Antibody SKU:CAB14996
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human LDLR (NP_000518.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa, Jurkat, LO2, HL-60, NIH/3T3
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 800 to the C-terminus of human LDLR (NP_000518.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:VFLC LGVF LLWK NWRL KNIN SINF DNPV YQKT TEDE VHIC HNQD GYSY PSRQ MVSL EDDV A
Gene ID:3949
Uniprot:P01130
Cellular Location:Cell membrane, Cell surface, Early endosome, Endomembrane system, Golgi apparatus, Late endosome, Lysosome, Membrane, Single-pass type I membrane protein, clathrin-coated pit
Calculated MW:75kDa/76kDa/82kDa/90kDa/95kDa
Observed MW:120kDa
Synonyms:LDLR, FH, FHC, LDLCQ2
Background:The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.
UniProt Protein Function:LDLR: Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. In order to be internalized, the receptor-ligand complexes must first cluster into clathrin-coated pits. In case of HIV-1 infection, functions as a receptor for extracellular Tat in neurons, mediating its internalization in uninfected cells. Defects in LDLR are the cause of familial hypercholesterolemia (FH); a common autosomal semi- dominant disease that affects about 1 in 500 individuals. The receptor defect impairs the catabolism of LDL, and the resultant elevation in plasma LDL-cholesterol promotes deposition of cholesterol in the skin (xanthelasma), tendons (xanthomas), and coronary arteries (atherosclerosis). Belongs to the LDLR family. 4 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Receptor, misc.; Membrane protein, integral; Cell surface

Chromosomal Location of Human Ortholog: 19p13.2

Cellular Component: apical part of cell; basolateral plasma membrane; cell surface; coated pit; early endosome; endosome membrane; external side of plasma membrane; Golgi apparatus; integral to plasma membrane; late endosome; lysosome; membrane; plasma membrane; receptor complex

Molecular Function:clathrin heavy chain binding; glycoprotein binding; identical protein binding; low-density lipoprotein receptor activity; protease binding; protein binding; receptor activity; very-low-density lipoprotein receptor activity

Biological Process: cholesterol absorption; cholesterol homeostasis; cholesterol transport; endocytosis; lipid metabolic process; phospholipid transport; receptor-mediated endocytosis

Disease: Hypercholesterolemia, Familial

NCBI Summary:The low density lipoprotein receptor (LDLR) gene family consists of cell surface proteins involved in receptor-mediated endocytosis of specific ligands. Low density lipoprotein (LDL) is normally bound at the cell membrane and taken into the cell ending up in lysosomes where the protein is degraded and the cholesterol is made available for repression of microsomal enzyme 3-hydroxy-3-methylglutaryl coenzyme A (HMG CoA) reductase, the rate-limiting step in cholesterol synthesis. At the same time, a reciprocal stimulation of cholesterol ester synthesis takes place. Mutations in this gene cause the autosomal dominant disorder, familial hypercholesterolemia. Alternate splicing results in multiple transcript variants.[provided by RefSeq, Sep 2010]
UniProt Code:P01130
NCBI GenInfo Identifier:126073
NCBI Gene ID:3949
NCBI Accession:P01130.1
UniProt Secondary Accession:P01130,Q53ZD9, Q59FQ1, Q9UDH7, B4DII3, B4DJZ8, B4DR00 B4DTQ3, C0JYY8, H0YLU8, H0YNT7,
UniProt Related Accession:P01130
Molecular Weight:82,255 Da
NCBI Full Name:Low-density lipoprotein receptor
NCBI Synonym Full Names:low density lipoprotein receptor
NCBI Official Symbol:LDLR  
NCBI Official Synonym Symbols:FH; FHC; LDLCQ2  
NCBI Protein Information:low-density lipoprotein receptor
UniProt Protein Name:Low-density lipoprotein receptor
Protein Family:LDLR chaperone
UniProt Gene Name:LDLR  
UniProt Entry Name:LDLR_HUMAN
Anti-LDLR Antibody (CAB14996)Western blot analysis of extracts of various cell lines, using LDLR antibody (CAB14996) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
Anti-LDLR Antibody (CAB14996)Immunohistochemistry of paraffin-embedded Mouse lung using LDLR antibody (CAB14996) at dilution of 1:100 (40x lens).
Anti-LDLR Antibody (CAB14996)Immunohistochemistry of paraffin-embedded Rat kidney using LDLR antibody (CAB14996) at dilution of 1:100 (40x lens).
Anti-LDLR Antibody (CAB14996)Immunofluorescence analysis of NIH/3T3 cells using LDLR antibody (CAB14996) at dilution of 1:50. Blue: DAPI for nuclear staining.
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