Name: Anti-LC3B Antibody [KO Validated] (CAB19665)
Brand: KO Validated Antibodies 2
SKU: CAB19665
Availability: OutOfStock

Antibody Name:	Anti-LC3B Antibody [KO Validated]
Antibody SKU:	CAB19665
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide of human LC3B.

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa, 293T, C6, NIH/3T3

Immunogen:	A synthetic peptide of human LC3B.
Purification Method:	Affinity purification
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	Email for sequence
Gene ID:	81631
Uniprot:	Q9GZQ8
Cellular Location:	Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:	14kDa, 16kDa
Observed MW:	14kDa, 16kDa

	Western blot analysis of extracts of various cell lines, using LC3B antibody (CAB19665) at 1:1000 dilution.293T cells, LC3B knockout (KO) 293T cells, C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts of Hela cells, using LC3B pAb (CAB19665) at 1:1000 dilution.Hela cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Basic Kit (RM00020). Exposure time: 5s.
	Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (CAB19665) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.
	Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (CAB19665) at 1:1000 dilution.293T cells were treated by Chloroquine (50 uM) at 37'C for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded Rat brain using LC3B antibody (CAB19665) at dilution of 1:100 (40x lens).

Synonyms:	LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1LC3B, LC3A/LC3B
Background:	The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.

NCBI Summary:	The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:	Q9GZQ8
NCBI GenInfo Identifier:	17433141
NCBI Gene ID:	81631
NCBI Accession:	Q9GZQ8.3
UniProt Related Accession:	Q9GZQ8
Molecular Weight:
NCBI Full Name:	Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:	microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:	MAP1LC3B
NCBI Official Synonym Symbols:	LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3
NCBI Protein Information:	microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:	Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:	Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:	MAP1LC3B
UniProt Entry Name:	MLP3B_HUMAN

Antibodies	ELISA Kits
Anti-LC3B Antibody (CAB11280)	Human LC3B / MAP1LC3B ELISA Kit
Anti-LC3B Antibody (CAB11282)[KO Validated]	Human Microtubule-associated proteins 1A/1B light chain 3B (MAP1LC3B) ELISA Kit
Anti-LC3B Antibody (CAB15592)
Anti-MAP1LC3B Mouse Monoclonal Antibody (CAB17424)
Anti-LC3B Antibody (CAB5601)