Autophagy Antibodies

Anti-LC3A / LC3B Antibody (CAB15591)

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SKU:
CAB15591
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Autophagy

Description

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Antibody Name:Anti-LC3A / LC3B Antibody
Antibody SKU:CAB15591
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse
Positive Samples:HeLa, NIH/3T3
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-125 of human LC3A / LC3B (NP_073729.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MPSE KTFK QRRT FEQR VEDV RLIR EQHP TKIP VIIE RYKG EKQL PVLD KTKF LVPD HVNM SELI KIIR RRLQ LNAN QAFF LLVN GHSM VSVS TPIS EVYE SEKD EDGF LYMV YASQ ETFG MKLS V
Gene ID:84557/81631
Uniprot:Q9GZQ8/Q9H492
Cellular Location:Cytoplasm, Cytoplasmic vesicle, Endomembrane system, Lipid-anchor, autophagosome, autophagosome membrane, cytoskeleton
Calculated MW:14kDa
Observed MW:14kDa, 16kDa
Synonyms:MAP1LC3B, ATG8F, LC3B, MAP1A/1BLC3, MAP1LC3B-a, MAP1LC3A, ATG8E, LC3, LC3A, MAP1ALC3, MAP1BLC3, LC3A/LC3B
Background: The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
NCBI Summary:The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component. [provided by RefSeq, Jul 2008]
UniProt Code:Q9GZQ8
NCBI GenInfo Identifier:17433141
NCBI Gene ID:81631
NCBI Accession:Q9GZQ8.3
UniProt Related Accession:Q9GZQ8
Molecular Weight:
NCBI Full Name:Microtubule-associated proteins 1A/1B light chain 3B
NCBI Synonym Full Names:microtubule associated protein 1 light chain 3 beta
NCBI Official Symbol:MAP1LC3B  
NCBI Official Synonym Symbols:LC3B; ATG8F; MAP1LC3B-a; MAP1A/1BLC3  
NCBI Protein Information:microtubule-associated proteins 1A/1B light chain 3B
UniProt Protein Name:Microtubule-associated proteins 1A/1B light chain 3B
UniProt Synonym Protein Names:Autophagy-related protein LC3 B; Autophagy-related ubiquitin-like modifier LC3 B; MAP1 light chain 3-like protein 2; MAP1A/MAP1B light chain 3 B; MAP1A/MAP1B LC3 B; Microtubule-associated protein 1 light chain 3 beta
UniProt Gene Name:MAP1LC3B  
UniProt Entry Name:MLP3B_HUMAN
Anti-LC3A / LC3B Antibody (CAB15591)Western blot analysis of extracts of various cell lines, using LC3A/LC3B antibody (CAB15591) at 1:1000 dilution. Hela cells were treated by Chloroquine (50 uM) for 20 hours. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 60s.
Anti-LC3A / LC3B Antibody (CAB15591)Immunohistochemistry of paraffin-embedded human breast cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).
Anti-LC3A / LC3B Antibody (CAB15591)Immunohistochemistry of paraffin-embedded human gastric cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).
Anti-LC3A / LC3B Antibody (CAB15591)Immunohistochemistry of paraffin-embedded human mammary cancer using LC3A / LC3B antibody (CAB15591) at dilution of 1:100 (40x lens).
Anti-LC3A / LC3B Antibody (CAB15591)Immunofluorescence analysis of HeLa cells using LC3A / LC3B antibody (CAB15591) at dilution of 1:100. Hela cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.
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