Antibody Name:	Anti-L1CAM Antibody [KO Validated]
Antibody SKU:	CAB8555
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	SH-SY5Y, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1143-1257 of human L1CAM (NP_000416.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	IKRS KGGK YSVK DKED TQVD SEAR PMKD ETFG EYRS LESD NEEK AFGS SQPS LNGD IKPL GSDD SLAD YGGS VDVQ FNED GSFI GQYS GKKE KEAA GGND SSGA TSPI NPAV ALE
Gene ID:	3897
Uniprot:	P32004
Cellular Location:	Cell membrane, Cell projection, Single-pass type I membrane protein, growth cone
Calculated MW:	138kDa/139kDa/140kDa
Observed MW:	200kDa

Synonyms:

L1CAM, CAML1, CD171, HSAS, HSAS1, MASA, MIC5, N-CAM-L1, N-CAML1, NCAM-L1, S10, SPG1

Background:

The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons.

UniProt Protein Function:	Neural cell adhesion molecule involved in the dynamics of cell adhesion and in the generation of transmembrane signals at tyrosine kinase receptors. During brain development, critical in multiple processes, including neuronal migration, axonal growth and fasciculation, and synaptogenesis. In the mature brain, plays a role in the dynamics of neuronal structure and function, including synaptic plasticity.
NCBI Summary:	The protein encoded by this gene is an axonal glycoprotein belonging to the immunoglobulin supergene family. The ectodomain, consisting of several immunoglobulin-like domains and fibronectin-like repeats (type III), is linked via a single transmembrane sequence to a conserved cytoplasmic domain. This cell adhesion molecule plays an important role in nervous system development, including neuronal migration and differentiation. Mutations in the gene cause X-linked neurological syndromes known as CRASH (corpus callosum hypoplasia, retardation, aphasia, spastic paraplegia and hydrocephalus). Alternative splicing of this gene results in multiple transcript variants, some of which include an alternate exon that is considered to be specific to neurons. [provided by RefSeq, May 2013]
UniProt Code:	P32004
NCBI GenInfo Identifier:	1705571
NCBI Gene ID:	3897
NCBI Accession:	P32004.2
UniProt Secondary Accession:	P32004,Q8TA87, A0AV65, A4ZYW4, B2RMU7, G3XAF4,
UniProt Related Accession:	P32004
Molecular Weight:	138,908 Da
NCBI Full Name:	Neural cell adhesion molecule L1
NCBI Synonym Full Names:	L1 cell adhesion molecule
NCBI Official Symbol:	L1CAM
NCBI Official Synonym Symbols:	S10; HSAS; MASA; MIC5; SPG1; CAML1; CD171; HSAS1; N-CAML1; NCAM-L1; N-CAM-L1
NCBI Protein Information:	neural cell adhesion molecule L1
UniProt Protein Name:	Neural cell adhesion molecule L1
UniProt Synonym Protein Names:	CD_antigen: CD171
Protein Family:	Neural cell adhesion molecule
UniProt Gene Name:	L1CAM

	Western blot analysis of extracts of various cell lines, using L1CAM antibody (CAB8555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Western blot analysis of extracts from normal (control) and L1CAM knockout (KO) HeLa cells, using L1CAM antibody (CAB8555) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.

Antibodies	ELISA Kits
Phospho-L1CAM (S1181) Antibody (PACO04186)	Human CAML1 / CD171 ELISA Kit
L1CAM Antibody (PACO06672)	Human L1CAM/CD171 (L1-Cell Adhesion Molecule) CLIA Kit (HUES01044)
L1CAM Antibody (PACO07368)	Human Neural cell adhesion molecule L1 (L1CAM) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)