Antibody Name:	Anti-KRT7 Antibody
Antibody SKU:	CAB2574
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 230-469 of human KRT7 (NP_005547.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ETEL TELQ SQIS DTSV VLSM DNSR SLDL DGII AEVK AQYE EMAK CSRA EAEA WYQT KFET LQAQ AGKH GDDL RNTR NEIS EMNR AIQR LQAE IDNI KNQR AKLE AAIA EAEE RGEL ALKD ARAK QEEL EAAL QRGK QDMA RQLR EYQE LMSV KLAL DIEI ATYR KLLE GEES RLAG DGVG AVNI SVMN STGG SSSG GGIG LTLG GTMG SNAL SFSS SAGP GLLK AYSI RTAS ASRR SARD
Gene ID:	3855
Uniprot:	P08729
Cellular Location:	Cytoplasm
Calculated MW:	51kDa
Observed MW:	51kDa

Synonyms:

KRT7, CK7, K2C7, K7, SCL, keratin 7, Keratin

Background:

The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described.

UniProt Protein Function:	K7: a type II cytoskeletal keratin. The keratins are intermediate filament proteins responsible for the structural integrity of epithelial cells and are subdivided into cytokeratins and hair keratins. Phosphorylation of keratins at specific sites affects their organization, assembly dynamics, and their interaction with signaling molecules. Specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels.
UniProt Protein Details:	Protein type:Cytoskeletal Chromosomal Location of Human Ortholog: 12q13.13 Cellular Component: cytoplasm; keratin filament; intermediate filament; nucleus Molecular Function:protein binding; structural molecule activity Biological Process: viral reproduction
NCBI Summary:	The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in the simple epithelia lining the cavities of the internal organs and in the gland ducts and blood vessels. The genes encoding the type II cytokeratins are clustered in a region of chromosome 12q12-q13. Alternative splicing may result in several transcript variants; however, not all variants have been fully described. [provided by RefSeq, Jul 2008]
UniProt Code:	P08729
NCBI GenInfo Identifier:	67782365
NCBI Gene ID:	3855
NCBI Accession:	NP_005547.3
UniProt Secondary Accession:	P08729,Q92676, Q9BUD8, Q9Y3R7,
UniProt Related Accession:	P08729
Molecular Weight:	51,386 Da
NCBI Full Name:	keratin, type II cytoskeletal 7
NCBI Synonym Full Names:	keratin 7
NCBI Official Symbol:	KRT7
NCBI Official Synonym Symbols:	K7; CK7; SCL; K2C7
NCBI Protein Information:	keratin, type II cytoskeletal 7; CK-7; keratin-7; sarcolectin; cytokeratin 7; cytokeratin-7; type-II keratin Kb7; type II mesothelial keratin K7; keratin, 55K type II cytoskeletal; keratin, simple epithelial type I, K7
UniProt Protein Name:	Keratin, type II cytoskeletal 7
UniProt Synonym Protein Names:	Cytokeratin-7; CK-7; Keratin-7; K7; Sarcolectin; Type-II keratin Kb7
Protein Family:	Keratin
UniProt Gene Name:	KRT7
UniProt Entry Name:	K2C7_HUMAN

	Western blot analysis of extracts of HeLa cells, using KRT7 antibody (CAB2574). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunohistochemistry of paraffin-embedded human placenta using KRT7 antibody (CAB2574) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of human lung cancer cells using KRT7 antibody (CAB2574) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of mouse bronchus cells using KRT7 antibody (CAB2574) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of rat bronchus cells using KRT7 antibody (CAB2574) at dilution of 1:100. Blue: DAPI for nuclear staining.