Cell Biology Antibodies 2

Anti-KRT4 Antibody (CAB12156)

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SKU:
CAB12156
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-KRT4 Antibody
Antibody SKU:CAB12156
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 446-520 of human KRT4 (NP_002263.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human
Positive Samples:BxPC-3, A-431
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 446-520 of human KRT4 (NP_002263.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:EEYR MSGE CQSA VSIS VVSG STST GGIS GGLG SGSG FGLS SGFG SGSG SGFG FGGS VSGS SSSK IIST TTLN KRR
Gene ID:3851
Uniprot:P19013
Cellular Location:
Calculated MW:57kDa
Observed MW:57kDa
Synonyms:KRT4, CK-4, CK4, CYK4, K4, WSN1, keratin 4
Background:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13.
UniProt Protein Function:K4: Heterotetramer of two type I and two type II keratins. Keratin-4 is generally associated with keratin-13. Detected in the suprabasal layer of the stratified epithelium of the esophagus, exocervix, vagina, mouth and lingual mucosa, and in cells and cell clusters in the mucosa and serous gland ducts of the esophageal submucosa. Expressed widely in the exocervix and esophageal epithelium, with lowest levels detected in the basal cell layer. Belongs to the intermediate filament family.
UniProt Protein Details:

Protein type:Cytoskeletal

Chromosomal Location of Human Ortholog: 12q13.13

Cellular Component: intermediate filament cytoskeleton; cell surface; keratin filament; intermediate filament; nucleus

Molecular Function:protein binding; structural molecule activity

Biological Process: epithelial cell differentiation; cytoskeleton organization and biogenesis; negative regulation of epithelial cell proliferation

Disease: White Sponge Nevus 1

NCBI Summary:The protein encoded by this gene is a member of the keratin gene family. The type II cytokeratins consist of basic or neutral proteins which are arranged in pairs of heterotypic keratin chains coexpressed during differentiation of simple and stratified epithelial tissues. This type II cytokeratin is specifically expressed in differentiated layers of the mucosal and esophageal epithelia with family member KRT13. Mutations in these genes have been associated with White Sponge Nevus, characterized by oral, esophageal, and anal leukoplakia. The type II cytokeratins are clustered in a region of chromosome 12q12-q13. [provided by RefSeq, Jul 2008]
UniProt Code:P19013
NCBI GenInfo Identifier:82654947
NCBI Gene ID:3851
NCBI Accession:P19013.4
UniProt Related Accession:P19013
Molecular Weight:
NCBI Full Name:Keratin, type II cytoskeletal 4
NCBI Synonym Full Names:keratin 4
NCBI Official Symbol:KRT4  
NCBI Official Synonym Symbols:K4; CK4; CK-4; CYK4; WSN1  
NCBI Protein Information:keratin, type II cytoskeletal 4
UniProt Protein Name:Keratin, type II cytoskeletal 4
UniProt Synonym Protein Names:Cytokeratin-4; CK-4; Keratin-4; K4; Type-II keratin Kb4
UniProt Gene Name:KRT4  
UniProt Entry Name:K2C4_HUMAN
Anti-KRT4 Antibody (CAB12156)Western blot analysis of extracts of various cell lines, using KRT4 antibody (CAB12156) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 15s.
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