Name: Anti-KPNB1 Antibody (CAB8610)
Brand: Immunology Antibodies 3
SKU: CAB8610
Availability: OutOfStock

Antibody Name:	Anti-KPNB1 Antibody
Antibody SKU:	CAB8610
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 250-350 of human KPNB1 (NP_002256.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, U-87MG, MCF7, HeLa, HepG2, mouse testis, mouse thymus, LO2, Mouse testis, Mouse lung, Mouse brain, Rat liver

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 250-350 of human KPNB1 (NP_002256.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	QYME TYMG PALF AITI EAMK SDID EVAL QGIE FWSN VCDE EMDL AIEA SEAA EQGR PPEH TSKF YAKG ALQY LVPI LTQT LTKQ DEND DDDD WNPC KAAG V
Gene ID:	3837
Uniprot:	Q14974
Cellular Location:	Cytoplasm, Nucleus envelope
Calculated MW:	81kDa/97kDa
Observed MW:	102kDa

Synonyms:

KPNB1, IMB1, IPO1, IPOB, Impnb, NTF97

Background:

Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus.
NCBI Summary:	Nucleocytoplasmic transport, a signal- and energy-dependent process, takes place through nuclear pore complexes embedded in the nuclear envelope. The import of proteins containing a nuclear localization signal (NLS) requires the NLS import receptor, a heterodimer of importin alpha and beta subunits also known as karyopherins. Importin alpha binds the NLS-containing cargo in the cytoplasm and importin beta docks the complex at the cytoplasmic side of the nuclear pore complex. In the presence of nucleoside triphosphates and the small GTP binding protein Ran, the complex moves into the nuclear pore complex and the importin subunits dissociate. Importin alpha enters the nucleoplasm with its passenger protein and importin beta remains at the pore. Interactions between importin beta and the FG repeats of nucleoporins are essential in translocation through the pore complex. The protein encoded by this gene is a member of the importin beta family. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Feb 2013]
UniProt Code:	Q14974
NCBI GenInfo Identifier:	20981701
NCBI Gene ID:	3837
NCBI Accession:	Q14974.2
UniProt Secondary Accession:	Q14974,Q14637, Q53XN2, Q96J27, B7ZAV6, D3DTT3,
UniProt Related Accession:	Q14974
Molecular Weight:	97 kDa
NCBI Full Name:	Importin subunit beta-1
NCBI Synonym Full Names:	karyopherin subunit beta 1
NCBI Official Symbol:	KPNB1
NCBI Official Synonym Symbols:	IMB1; IPO1; IPOB; Impnb; NTF97
NCBI Protein Information:	importin subunit beta-1
UniProt Protein Name:	Importin subunit beta-1
UniProt Synonym Protein Names:	Importin-90; Karyopherin subunit beta-1; Nuclear factor p97; Pore targeting complex 97 kDa subunit; PTAC97
Protein Family:	Importin
UniProt Gene Name:	KPNB1

Anti-KPNB1 Polyclonal Antibody (CAB8610)

Western blot analysis of extracts of various cell lines, using KPNB1 antibody (CAB8610) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.

Antibodies

KPNB1 Antibody (PACO10156)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)