KO Validated Antibodies 2

Anti-KLF4 Antibody (CAB18088)[KO Validated]

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SKU:
CAB18088
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KLF4 Antibody [KO Validated]
Antibody SKU:CAB18088
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 180-479 of human KLF4 (NP_004226.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:PPTA PFNL ADIN DVSP SGGF VAEL LRPE LDPV YIPP QQPQ PPGG GLMG KFVL KASL SAPG SEYG SPSV ISVS KGSP DGSH PVVV APYN GGPP RTCP KIKQ EAVS SCTH LGAG PPLS NGHR PAAH DFPL GRQL PSRT TPTL GLEE VLSS RDCH PALP LPPG FHPH PGPN YPSF LPDQ MQPQ VPPL HYQE LMPP GSCM PEEP KPKR GRRS WPRK RTAT HTCD YAGC GKTY TKSS HLKA HLRT HTGE KPYH CDWD GCGW KFAR SDEL TRHY RKHT GHRP FQCQ KCDR AFSR SDHL ALHM KRHF
Gene ID:9314
Uniprot:O43474
Cellular Location:Nucleus
Calculated MW:6kDa/12kDa/45kDa/51kDa/54kDa
Observed MW:62kDa
Anti-KLF4 Antibody [KO Validated] (CAB18088)Western blot analysis of extracts from normal (control) and KLF4 knockout (KO) HeLa cells, using KLF4 antibody (CAB18088) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
Synonyms:EZF, GKLF, KLF4
Background:This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is required for normal development of the barrier function of skin. The encoded protein is thought to control the G1-to-S transition of the cell cycle following DNA damage by mediating the tumor suppressor gene p53. Mice lacking this gene have a normal appearance but lose weight rapidly, and die shortly after birth due to fluid evaporation resulting from compromised epidermal barrier function. Alternative splicing results in multiple transcript variants encoding different isoforms.
UniProt Code:O43474
NCBI GenInfo Identifier:194248077
NCBI Gene ID:9314
NCBI Accession:NP_004226.3
UniProt Secondary Accession:O43474,P78338, Q5T3J8, Q5T3J9, Q8N717, Q9UNP3, B2R8S4 B3KT79, L0R3I6, L0R4N5,
UniProt Related Accession:O43474
Molecular Weight:51kDa
NCBI Full Name:Krueppel-like factor 4
NCBI Synonym Full Names:Kruppel-like factor 4 (gut)
NCBI Official Symbol:KLF4  
NCBI Official Synonym Symbols:EZF; GKLF  
NCBI Protein Information:Krueppel-like factor 4; gut-enriched krueppel-like factor; epithelial zinc finger protein EZF; endothelial Kruppel-like zinc finger protein
UniProt Protein Name:Krueppel-like factor 4
UniProt Synonym Protein Names:Epithelial zinc finger protein EZF; Gut-enriched krueppel-like factor
Protein Family:Krueppel-like factor
UniProt Gene Name:KLF4  
UniProt Entry Name:KLF4_HUMAN
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