Antibody Name:	Anti-KLC1 Antibody
Antibody SKU:	CAB5552
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-560 of human KLC1 (NP_005543.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, 22Rv1, BT-474, MCF7, Mouse brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 261-560 of human KLC1 (NP_005543.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	LALV YRDQ NKYK DAAN LLND ALAI REKT LGKD HPAV AATL NNLA VLYG KRGK YKEA EPLC KRAL EIRE KVLG KDHP DVAK QLNN LALL CQNQ GKYE EVEY YYQR ALEI YQTK LGPD DPNV AKTK NNLA SCYL KQGK FKQA ETLY KEIL TRAH EREF GSVD DENK PIWM HAEE REEC KGKQ KDGT SFGE YGGW YKAC KVDS PTVT TTLK NLGA LYRR QGKF EAAE TLEE AAMR SRKQ GLDN VHKQ RVAE VLND PENM EKRR SRES LNVD VVKY ESGP DGGE EVSM SVEW NGMR KMKL GLVN
Gene ID:	3831
Uniprot:	Q07866
Cellular Location:	Cell projection, Cytoplasm, Cytoplasmic vesicle, cytoskeleton, growth cone
Calculated MW:	62-72kDa
Observed MW:	60-75kDa

Synonyms:

KLC1, KLC, KNS2, KNS2A

Background:

Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named 'kinesin 2', this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined.

UniProt Protein Function:	KLC1: a microtubule-associated force-producing protein that may play a role in organelle transport. The light chain may function in coupling of cargo to the heavy chain or in the modulation of its ATPase activity.
UniProt Protein Details:	Protein type:Motor; Microtubule-binding Chromosomal Location of Human Ortholog: 14q32.3 Cellular Component: ciliary rootlet; kinesin complex; microtubule; growth cone; membrane; cell soma; axon; cytoplasmic membrane-bound vesicle; cytosol Molecular Function:tubulin binding; protein binding; motor activity; microtubule motor activity Biological Process: intracellular protein transport; viral reproduction; metabolic process; axon cargo transport; antigen processing and presentation of exogenous peptide antigen via MHC class II; blood coagulation; microtubule-based movement
NCBI Summary:	Conventional kinesin is a tetrameric molecule composed of two heavy chains and two light chains, and transports various cargos along microtubules toward their plus ends. The heavy chains provide the motor activity, while the light chains bind to various cargos. This gene encodes a member of the kinesin light chain family. It associates with kinesin heavy chain through an N-terminal domain, and six tetratricopeptide repeat (TPR) motifs are thought to be involved in binding of cargos such as vesicles, mitochondria, and the Golgi complex. Thus, kinesin light chains function as adapter molecules and not motors per se. Although previously named "kinesin 2", this gene is not a member of the kinesin-2 / kinesin heavy chain subfamily of kinesin motor proteins. Extensive alternative splicing produces isoforms with different C-termini that are proposed to bind to different cargos; however, the full-length nature and/or biological validity of most of these variants have not been determined. [provided by RefSeq, Jul 2008]
UniProt Code:	Q07866
NCBI GenInfo Identifier:	223590110
NCBI Gene ID:	3831
NCBI Accession:	Q07866.2
UniProt Secondary Accession:	Q07866,Q7RTM2, Q7RTM3, Q7RTM5, Q7RTP9, Q7RTQ3, Q7RTQ5 Q7RTQ6, Q86SF5, Q86TF5, A6NF62, F8VTM4,
UniProt Related Accession:	Q07866
Molecular Weight:	573
NCBI Full Name:	Kinesin light chain 1
NCBI Synonym Full Names:	kinesin light chain 1
NCBI Official Symbol:	KLC1
NCBI Official Synonym Symbols:	KLC; KNS2; KNS2A
NCBI Protein Information:	kinesin light chain 1; kinesin 2 60/70kDa; medulloblastoma antigen MU-MB-2.50
UniProt Protein Name:	Kinesin light chain 1
Protein Family:	Kinesin
UniProt Gene Name:	KLC1
UniProt Entry Name:	KLC1_HUMAN

	Western blot analysis of extracts of various cell lines, using KLC1 antibody (CAB5552) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.
	Immunofluorescence analysis of U2OS cells using KLC1 antibody (CAB5552). Blue: DAPI for nuclear staining.