Antibody Name:	Anti-KIR3DS1 Antibody
Antibody SKU:	CAB9936
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human KIR3DS1 (NP_001077008.1).

Application:	WB IHC
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Jurkat, A-549, THP-1, BT-474, B cells, Mouse liver, Mouse kidney, Mouse pancreas, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 80-340 of human KIR3DS1 (NP_001077008.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FNMS PVTT AHAG NYTC RGSH PHSP TGWS APSN PMVI MVTG NHRK PSLL AHPG PLVK SGER VILQ CWSD IMFE HFFL HKEW ISKD PSRL VGQI HDGV SKAN FSIG SMMR ALAG TYRC YGSV THTP YQLS APSD PLDI VVTG LYEK PSLS AQPG PKVQ AGES VTLS CSSR SSYD MYHL SREG GAHE RRLP AVRK VNRT FQAD FPLG PATH GGTY RCFG SFRH SPYE WSDP SDPL LVSV TGNP SSSW PSPT EPSS KSGN LRHL H
Gene ID:	3813
Uniprot:	Q14943
Cellular Location:	Cell membrane, Single-pass type I membrane protein
Calculated MW:	42kDa
Observed MW:	60kDa

Synonyms:

KIR3DS1, CD158E2, KIR-123FM, KIR-G1, NKAT-10, NKAT10

Background:

Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several 'framework' genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

UniProt Protein Function:	KIR3DS1: Receptor on natural killer (NK) cells for HLA-C alleles. Does not inhibit the activity of NK cells. Belongs to the immunoglobulin superfamily.
UniProt Protein Details:	Protein type:Membrane protein, integral Chromosomal Location of Human Ortholog: 19q13.4 Cellular Component: plasma membrane Biological Process: innate immune response
NCBI Summary:	Killer cell immunoglobulin-like receptors (KIRs) are transmembrane glycoproteins expressed by natural killer cells and subsets of T cells. The KIR genes are polymorphic and highly homologous and they are found in a cluster on chromosome 19q13.4 within the 1 Mb leukocyte receptor complex (LRC). The gene content of the KIR gene cluster varies among haplotypes, although several "framework" genes are found in all haplotypes (KIR3DL3, KIR3DP1, KIR3DL4, KIR3DL2). The KIR proteins are classified by the number of extracellular immunoglobulin domains (2D or 3D) and by whether they have a long (L) or short (S) cytoplasmic domain. KIR proteins with the long cytoplasmic domain transduce inhibitory signals upon ligand binding via an immune tyrosine-based inhibitory motif (ITIM), while KIR proteins with the short cytoplasmic domain lack the ITIM motif and instead associate with the TYRO protein tyrosine kinase binding protein to transduce activating signals. The ligands for several KIR proteins are subsets of HLA class I molecules; thus, KIR proteins are thought to play an important role in regulation of the immune response. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2013]
UniProt Code:	Q14943
NCBI GenInfo Identifier:	2833258
NCBI Gene ID:	3813
NCBI Accession:	Q14943.1
UniProt Related Accession:	Q14943
Molecular Weight:	42,902 Da
NCBI Full Name:	Killer cell immunoglobulin-like receptor 3DS1
NCBI Synonym Full Names:	killer cell immunoglobulin like receptor, three Ig domains and short cytoplasmic tail 1
NCBI Official Symbol:	KIR3DS1
NCBI Official Synonym Symbols:	KIR-G1; NKAT10; CD158E2; NKAT-10; KIR-123FM
NCBI Protein Information:	killer cell immunoglobulin-like receptor 3DS1
UniProt Protein Name:	Killer cell immunoglobulin-like receptor 3DS1
UniProt Synonym Protein Names:	MHC class I NK cell receptor; Natural killer-associated transcript 10; NKAT-10
Protein Family:	Killer cell immunoglobulin-like receptor
UniProt Gene Name:	KIR3DS1
UniProt Entry Name:	KI3S1_HUMAN

	Western blot analysis of extracts of various cell lines, using KIR3DS1 antibody (CAB9936) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
	Immunohistochemistry of paraffin-embedded human liver using KIR3DS1 antibody (CAB9936) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human mammary cancer using KIR3DS1 antibody (CAB9936) at dilution of 1:100 (40x lens).