KO Validated Antibodies 1

Anti-KDM1 Antibody (CAB1156)[KO Validated]

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SKU:
CAB1156
Product Type:
Antibody
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Reactivity:
Human, Mouse, Rat
Synonyms:
AOF2, CPRF, KDM1, LSD1, BHC110
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] KDM1 Antibody (CAB1156)

The [KO Validated] KDM1 Antibody (CAB1156) is a high-quality antibody developed for reliable detection and analysis of target proteins. This polyclonal antibody, generated in rabbits, is highly specific and can reliably detect KDM1A in human samples. It has been rigorously validated for use in Western blotting applications, enabling precise and accurate analysis of KDM1A expression levels in various cell types.KDM1A, also known as lysine-specific histone demethylase 1A, plays a vital role in epigenetic regulation by removing methyl groups from histone proteins.

This antibody is validated for use in WB, IP, ELISA applications and has demonstrated reactivity against Human, Mouse, Rat samples.

Product Name:[KO Validated] KDM1 Antibody
SKU:CAB1156
Size:20μL, 100μL
Reactivity:Human, Mouse, Rat
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:LSED EYYS EEER NAKA EKEK KLPP PPPQ APPE EENE SEPE EPSG VEGA AFQS RLPH DRMT SQEA ACFP DIIS GPQQ TQKV FLFI RNRT LQLW LDNP KIQL TFEA TLQQ LEAP YNSD TVLV HRVH SYLE RHGL INFG IYKR IKPL PTKK TGKV IIIG SGVS GLAA ARQL QSFG MDVT LLEA RDRV GGRV ATFR KGNY VADL GAMV VTGL GGNP MAVV SKQV NMEL AKIK QKCP LYEA NGQA VPKE KDEM VEQ
Tested Applications:WB IP ELISA
Recommended Dilution:
WB1:500 - 1:2000
IP0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:AOF2, CPRF, KDM1, LSD1, BHC110
Positive Sample:MCF7, 293T
Cellular Localization:Nucleus.
Calculated MW:93kDa
Observed MW:117kDa

This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID23028
RRIDAB_2721240
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide,50% glycerol,pH7.3.
Product ImageWestern blot analysis of lysates from MCF7 cells, using KDM1 Rabbit pAb (CAB1156) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020).
Product ImageImmunoprecipitation analysis of 200 μg extracts of HeLa cells using 3 μg KDM1 antibody (CAB1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (CAB1156) at a dilution of 1:500.
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