KO Validated Antibodies 1

Anti-KDM1 Antibody (CAB1156)[KO Validated]

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SKU:
CAB1156
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-KDM1 Antibody [KO Validated]
Antibody SKU:CAB1156
Antibody Size:20uL, 50uL, 100uL
Application:WB IF IP
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Application:WB IF IP
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:100
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:LSED EYYS EEER NAKA EKEK KLPP PPPQ APPE EENE SEPE EPSG VEGA AFQS RLPH DRMT SQEA ACFP DIIS GPQQ TQKV FLFI RNRT LQLW LDNP KIQL TFEA TLQQ LEAP YNSD TVLV HRVH SYLE RHGL INFG IYKR IKPL PTKK TGKV IIIG SGVS GLAA ARQL QSFG MDVT LLEA RDRV GGRV ATFR KGNY VADL GAMV VTGL GGNP MAVV SKQV NMEL AKIK QKCP LYEA NGQA VPKE KDEM VEQ
Gene ID:23028
Uniprot:O60341
Cellular Location:Nucleus
Calculated MW:92kDa/95kDa
Observed MW:117kDa
Synonyms:KDM1A, AOF2, BHC110, CPRF, KDM1, LSD1, KDM1
Background:This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
UniProt Code:O60341
NCBI GenInfo Identifier:58761544
NCBI Gene ID:23028
NCBI Accession:NP_001009999.1
UniProt Secondary Accession:O60341,Q5TH94, Q5TH95, Q86VT7, Q8IXK4, Q8NDP6, Q8TAZ3 Q96AW4, A8MWP9,
UniProt Related Accession:O60341
Molecular Weight:852
NCBI Full Name:lysine-specific histone demethylase 1A isoform a
NCBI Synonym Full Names:lysine (K)-specific demethylase 1A
NCBI Official Symbol:KDM1A  
NCBI Official Synonym Symbols:AOF2; KDM1; LSD1; BHC110  
NCBI Protein Information:lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; amine oxidase (flavin containing) domain 2; FAD-binding protein BRAF35-HDAC complex, 110 kDa subunit; flavin-containing amine oxidase domain-con
UniProt Protein Name:Lysine-specific histone demethylase 1A
UniProt Synonym Protein Names:BRAF35-HDAC complex protein BHC110; Flavin-containing amine oxidase domain-containing protein 2
Protein Family:Lysine-specific histone demethylase
UniProt Gene Name:KDM1A  
UniProt Entry Name:KDM1A_HUMAN
Anti-KDM1 Antibody [KO Validated] (CAB1156)Western blot analysis of extracts of MCF7 cells, using KDM1 antibody (CAB1156) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020).
Anti-KDM1 Antibody [KO Validated] (CAB1156)Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody (CAB1156) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-KDM1 Antibody [KO Validated] (CAB1156)Immunofluorescence analysis of A549 cells using KDM1 antibody (CAB1156).
Anti-KDM1 Antibody [KO Validated] (CAB1156)Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1 antibody (CAB1156). Western blot was performed from the immunoprecipitate using KDM1 antibody (CAB1156) at a dilition of 1:500.
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