Signal Transduction Antibodies 3

Anti-KCNJ5 Antibody (CAB6232)

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SKU:
CAB6232
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-KCNJ5 Antibody
Antibody SKU:CAB6232
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KCNJ5 (NP_000881.3).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse pancreas, Rat heart
Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KCNJ5 (NP_000881.3).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:RQRY MEKS GKCN VHHG NVQE TYRY LSDL FTTL VDLK WRFN LLVF TMVY TVTW LFFG FIWW LIAY IRGD LDHV GDQE WIPC VENL SGFV SAFL FSIE TETT I
Gene ID:3762
Uniprot:P48544
Cellular Location:Membrane, Multi-pass membrane protein
Calculated MW:47kDa
Observed MW:47kDa
Synonyms:KCNJ5, CIR, GIRK4, KATP1, KIR3.4, LQT13
Background:Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It may associate with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex.
UniProt Protein Function:GIRK4: This potassium channel is controlled by G proteins. Inward rectifier potassium channels are characterized by a greater tendency to allow potassium to flow into the cell rather than out of it. Their voltage dependence is regulated by the concentration of extracellular potassium; as external potassium is raised, the voltage range of the channel opening shifts to more positive voltages. The inward rectification is mainly due to the blockage of outward current by internal magnesium. Can be blocked by external barium. Defects in KCNJ5 are the cause of long QT syndrome type 13 (LQT13). It is a heart disorder characterized by a prolonged QT interval on the ECG and polymorphic ventricular arrhythmias. They cause syncope and sudden death in response to excercise or emotional stress, and can present with a sentinel event of sudden cardiac death in infancy. Defects in KCNJ5 are the cause of familial hyperaldosteronism type 3 (FH3). A form of hyperaldosteronism characterized by hypertension secondary to massive adrenal mineralocorticoid production. Like patients with familial hyperaldosteronism type 1 (glucocorticoid-remediable aldosteronism), patients with FH3 present with childhood hypertension, elevated aldosteronism levels, and high levels of the hybrid steroids 18-oxocortisol and 18-hydroxycortisol. However, hypertension and aldosteronism are not reversed by administration of exogenous glucocorticoids and patients require adrenalectomy to control hypertension. Somatic mutations in KCNJ5 have been found in aldosterone-producing adrenal adenomas and can be responsible for aldosteronism associated with cell autonomous proliferation. These are typically solitary, well circumscribed tumors diagnosed between ages 30 and 70. They come to medical attention due to new or worsening hypertension, often with hypokalemia. KCNJ5 mutations produce increased sodium conductance and cell depolarization, which in adrenal glomerulosa cells produces calcium entry, the signal for aldosterone production and cell proliferation. Belongs to the inward rectifier-type potassium channel (TC 1.A.2.1) family. KCNJ5 subfamily.
UniProt Protein Details:

Protein type:Membrane protein, multi-pass; Membrane protein, integral; Channel, potassium

Chromosomal Location of Human Ortholog: 11q24

Cellular Component: voltage-gated potassium channel complex; plasma membrane

Molecular Function:protein binding; G-protein activated inward rectifier potassium channel activity; inward rectifier potassium channel activity

Biological Process: synaptic transmission; potassium ion import; potassium ion transport

Disease: Hyperaldosteronism, Familial, Type Iii; Long Qt Syndrome 13

NCBI Summary:Potassium channels are present in most mammalian cells, where they participate in a wide range of physiologic responses. The protein encoded by this gene is an integral membrane protein and inward-rectifier type potassium channel. The encoded protein, which has a greater tendency to allow potassium to flow into a cell rather than out of a cell, is controlled by G-proteins. It may associate with two other G-protein-activated potassium channels to form a heteromultimeric pore-forming complex. [provided by RefSeq, Jul 2008]
UniProt Code:P48544
NCBI GenInfo Identifier:296434543
NCBI Gene ID:3762
NCBI Accession:P48544.2
UniProt Secondary Accession:P48544,Q6DK13, Q6DK14, Q92807, B2R744,
UniProt Related Accession:P48544
Molecular Weight:419
NCBI Full Name:G protein-activated inward rectifier potassium channel 4
NCBI Synonym Full Names:potassium inwardly-rectifying channel, subfamily J, member 5
NCBI Official Symbol:KCNJ5  
NCBI Official Synonym Symbols:CIR; GIRK4; KATP1; LQT13; KIR3.4  
NCBI Protein Information:G protein-activated inward rectifier potassium channel 4; IRK-4; heart KATP channel; inward rectifier K+ channel KIR3.4; cardiac ATP-sensitive potassium channel
UniProt Protein Name:G protein-activated inward rectifier potassium channel 4
UniProt Synonym Protein Names:Cardiac inward rectifier; CIR; Heart KATP channel; Inward rectifier K(+) channel Kir3.4; IRK-4; KATP-1; Potassium channel, inwardly rectifying subfamily J member 5
Protein Family:G protein-activated inward rectifier potassium channel
UniProt Gene Name:KCNJ5  
UniProt Entry Name:KCNJ5_HUMAN
Anti-KCNJ5 Antibody (CAB6232)Western blot analysis of extracts of various cell lines, using KCNJ5 Antibody (CAB6232) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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