Name: Anti-KCNH1 Antibody (CAB6636)
Brand: Signal Transduction Antibodies 3
SKU: CAB6636
Availability: OutOfStock

Antibody Name:	Anti-KCNH1 Antibody
Antibody SKU:	CAB6636
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 813-962 of human KCNH1 (NP_002229.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	Raji, MCF7, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 813-962 of human KCNH1 (NP_002229.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	FKDA CGKS EDWN KVSK AESM ETLP ERTK ASGE ATLK KTDS CDSG ITKS DLRL DNVG EARS PQDR SPIL AEVK HSFY PIPE QTLQ ATVL EVRH ELKE DIKA LNAK MTNI EKQL SEIL RILT SRRS SQSP QELF EISR PQSP ESER DIFG AS
Gene ID:	3756
Uniprot:	O95259
Cellular Location:	Cell membrane, Early endosome membrane, Multi-pass membrane protein, Nucleus inner membrane
Calculated MW:	108kDa/111kDa
Observed MW:	111kDa

Synonyms:

KCNH1, EAG, EAG1, Kv10.1, TMBTS, ZLS1, h-eag, hEAG1

Background:

Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms.

UniProt Protein Function:	Kv10.1: Pore-forming (alpha) subunit of voltage-gated non- inactivating delayed rectifier potassium channel. Channel properties may be modulated by cAMP and subunit assembly. Mediates IK(NI) current in myoblasts. Belongs to the potassium channel family. H (Eag) (TC 1.A.1.20) subfamily. Kv10.1/KCNH1 sub-subfamily. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 1q32.2 Cellular Component: nucleoplasm; voltage-gated potassium channel complex; integral to plasma membrane; early endosome membrane; cytoplasm; plasma membrane; nuclear inner membrane Molecular Function:calmodulin binding; protein binding; cyclic nucleotide binding; delayed rectifier potassium channel activity; two-component sensor activity Biological Process: synaptic transmission; regulation of membrane potential; two-component signal transduction system (phosphorelay); potassium ion transport; myoblast fusion; regulation of cell proliferation Disease: Zimmermann-laband Syndrome 1; Temple-baraitser Syndrome
NCBI Summary:	Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. This gene encodes a member of the potassium channel, voltage-gated, subfamily H. This member is a pore-forming (alpha) subunit of a voltage-gated non-inactivating delayed rectifier potassium channel. It is activated at the onset of myoblast differentiation. The gene is highly expressed in brain and in myoblasts. Overexpression of the gene may confer a growth advantage to cancer cells and favor tumor cell proliferation. Alternative splicing of this gene results in two transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]
UniProt Code:	O95259
NCBI GenInfo Identifier:	26006799
NCBI Gene ID:	3756
NCBI Accession:	O95259.1
UniProt Secondary Accession:	O95259,O76035, Q14CL3, B1AQ26,
UniProt Related Accession:	O95259
Molecular Weight:	989
NCBI Full Name:	Potassium voltage-gated channel subfamily H member 1
NCBI Synonym Full Names:	potassium voltage-gated channel, subfamily H (eag-related), member 1
NCBI Official Symbol:	KCNH1
NCBI Official Synonym Symbols:	EAG; EAG1; h-eag; Kv10.1
NCBI Protein Information:	potassium voltage-gated channel subfamily H member 1; hEAG1; EAG channel 1; ether-a-go-go potassium channel 1; ether-a-go-go, Drosophila, homolog of; voltage-gated potassium channel subunit Kv10.1
UniProt Protein Name:	Potassium voltage-gated channel subfamily H member 1
UniProt Synonym Protein Names:	Ether-a-go-go potassium channel 1; EAG channel 1; h-eag; hEAG1; Voltage-gated potassium channel subunit Kv10.1
Protein Family:	Potassium voltage-gated channel subfamily
UniProt Gene Name:	KCNH1
UniProt Entry Name:	KCNH1_HUMAN

Western blot analysis of extracts of various cell lines, using KCNH1 antibody (CAB6636) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.

Antibodies

KCNH1 Antibody (PACO00994)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)