Antibody Name:	Anti-KAT6A Antibody
Antibody SKU:	CAB15006
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 800-1000 of human KAT6A (NP_001092883.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 800-1000 of human KAT6A (NP_001092883.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	EEPQ CQER ELEI SVGK SVSH ENKE QDSY SVES EKKP EVMA PVSS TRLS KQVL PHDS LPAN SQPS RRGR WGRK NRKT QERF GDKD SKLL LEET SSAP QEQY GECG EKSE ATQE QYTE SEEQ LVAS EEQP SQDG KPDL PKRR LSEG VEPW RGQL KKSP EALK CRLT EGSE RLPR RYSE GDRA VLRG FSES SEEE EEPE SPRS S
Gene ID:	7994
Uniprot:	Q92794
Cellular Location:	Nucleus, PML body, nucleolus, nucleoplasm
Calculated MW:	225kDa
Observed MW:	250kDa

Synonyms:

KAT6A, MOZ, MRD32, MYST-3, MYST3, RUNXBP2, ZC2HC6A, ZNF220

Background:

This gene encodes a member of the MOZ, YBFR2, SAS2, TIP60 family of histone acetyltransferases. The protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain. It is part of a complex that acetylates lysine-9 residues in histone 3, and in addition, it acts as a co-activator for several transcription factors. Allelic variants of this gene are associated with autosomal dominant mental retardation-32. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	MYST3: Histone acetyltransferase that acetylates lysine residues in histone H3 and histone H4 (in vitro). Component of the MOZ/MORF complex which has a histone H3 acetyltransferase activity. May act as a transcriptional coactivator for RUNX1 and RUNX2. Chromosomal aberrations involving KAT6A may be a cause of acute myeloid leukemias. Translocation t(8;16)(p11;p13) with CREBBP; translocation t(8;22)(p11;q13) with EP300. KAT6A- CREBBP may induce leukemia by inhibiting RUNX1-mediated transcription. Inversion inv(8)(p11;q13) generates the KAT6A-NCOA2 oncogene, which consists of the N-terminal part of KAT6A and the C-terminal part of NCOA2/TIF2. KAT6A-NCOA2 binds to CREBBP and disrupts its function in transcription activation. A chromosomal aberration involving KAT6A is a cause of therapy-related myelodysplastic syndrome. Translocation t(2;8)(p23;p11.2) with ASXL2 generates a KAT6A-ASXL2 fusion protein. Belongs to the MYST (SAS/MOZ) family.
UniProt Protein Details:	Protein type:Acetyltransferase; EC 2.3.1.48; Oncoprotein; Nucleolus Chromosomal Location of Human Ortholog: 8p11 Cellular Component: Golgi apparatus; nucleoplasm; nucleus; PML body Molecular Function:acetyltransferase activity; DNA binding; histone acetyltransferase activity; protein binding; transcription coactivator activity; transcription factor binding; zinc ion binding Biological Process: DNA packaging; histone acetylation; myeloid cell differentiation; negative regulation of transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; protein amino acid acetylation Disease: Mental Retardation, Autosomal Dominant 32
NCBI Summary:	This gene encodes a member of the MOZ, YBFR2, SAS2, TIP60 family of histone acetyltransferases. The protein is composed of a nuclear localization domain, a double C2H2 zinc finger domain that binds to acetylated histone tails, a histone acetyl-transferase domain, a glutamate/aspartate-rich region, and a serine- and methionine-rich transactivation domain. It is part of a complex that acetylates lysine-9 residues in histone 3, and in addition, it acts as a co-activator for several transcription factors. Allelic variants of this gene are associated with autosomal dominant mental retardation-32. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Apr 2015]
UniProt Code:	Q92794
NCBI GenInfo Identifier:	215274095
NCBI Gene ID:	7994
NCBI Accession:	Q92794.2
UniProt Secondary Accession:	Q92794,Q76L81,
UniProt Related Accession:	Q92794
Molecular Weight:	225,028 Da
NCBI Full Name:	Histone acetyltransferase KAT6A
NCBI Synonym Full Names:	lysine acetyltransferase 6A
NCBI Official Symbol:	KAT6A
NCBI Official Synonym Symbols:	MOZ; MRD32; MYST3; MYST-3; ZNF220; RUNXBP2; ZC2HC6A
NCBI Protein Information:	histone acetyltransferase KAT6A
UniProt Protein Name:	Histone acetyltransferase KAT6A
UniProt Synonym Protein Names:	MOZ, YBF2/SAS3, SAS2 and TIP60 protein 3; MYST-3; Monocytic leukemia zinc finger protein; Runt-related transcription factor-binding protein 2; Zinc finger protein 220
Protein Family:	Histone acetyltransferase
UniProt Gene Name:	KAT6A
UniProt Entry Name:	KAT6A_HUMAN

	Western blot analysis of extracts of 293T cells, using KAT6A antibody (CAB15006) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunofluorescence analysis of NIH/3T3 cells using KAT6A antibody (CAB15006) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using KAT6A antibody (CAB15006) at dilution of 1:100. Blue: DAPI for nuclear staining.