Cell Biology Antibodies 11
Anti-JMJD7 Antibody (CAB7408)
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- SKU:
- CAB7408
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-JMJD7 Antibody |
| Antibody SKU: | CAB7408 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human JMJD7 (NP_001108104.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | MCF7, SKOV3, Mouse heart |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-316 of human JMJD7 (NP_001108104.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MAEA ALEA VRSE LREF PAAA RELC VPLA VPYL DKPP TPLH FYRD WVCP NRPC IIRN ALQH WPAL QKWS LPYF RATV GSTE VSVA VTPD GYAD AVRG DRFM MPAE RRLP LSFV LDVL EGRA QHPG VLYV QKQC SNLP SELP QLLP DLES HVPW ASEA LGKM PDAV NFWL GEAA AVTS LHKD HYEN LYCV VSGE KHFL FHPP SDRP FIPY ELYT PATY QLTE EGTF KVVD EEAM EKVP WIPL DPLA PDLA RYPS YSQA QALR CTVR AGEM LYLP ALWF HHVQ QSQG CIAV NFWY DMEY DLKY SYFQ LLDS LTKA SGLD |
| Gene ID: | 100137047 |
| Uniprot: | P0C870 |
| Cellular Location: | |
| Calculated MW: | 35kDa |
| Observed MW: | 36kDa |
| Synonyms: | JMJD7 |
| Background: | This gene encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein. |
| UniProt Protein Function: | JMJD7: a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein. [RefSeq, Jul 2008] |
| UniProt Protein Details: | Protein type:Unknown function Chromosomal Location of Human Ortholog: 15q15.1 |
| NCBI Summary: | This gene encodes a highly conserved protein with a JmjC domain, which are part of the cupin metalloenzyme superfamily. JmjC proteins may function as 2-oxoglutarate-Fe(II)-dependent dioxygenases. Most tissues also express read-through transcripts from this gene into the downstream phospholipase A2, group IVB (cytosolic) gene, some of which may encode fusion proteins combining the N-terminus of this protein with the phospholipase A2, group IVB protein. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P0C870 |
| NCBI GenInfo Identifier: | 205783894 |
| NCBI Gene ID: | 100137047 |
| NCBI Accession: | P0C870.1 |
| UniProt Secondary Accession: | P0C870,O95712, Q59GF9, Q8TB10, Q9UKV7, A5D6V5, |
| UniProt Related Accession: | P0C870 |
| Molecular Weight: | 35,932 Da |
| NCBI Full Name: | JmjC domain-containing protein 7 |
| NCBI Synonym Full Names: | jumonji domain containing 7 |
| NCBI Official Symbol: | JMJD7 |
| NCBI Protein Information: | jmjC domain-containing protein 7; jumonji domain-containing protein 7 |
| UniProt Protein Name: | JmjC domain-containing protein 7 |
| UniProt Synonym Protein Names: | Jumonji domain-containing protein 7 |
| Protein Family: | JmjC domain-containing protein |
| UniProt Gene Name: | JMJD7 |
| UniProt Entry Name: | JMJD7_HUMAN |
 | Western blot analysis of extracts of various cell lines, using JMJD7 antibody (CAB7408) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 90s. |
 | Immunohistochemistry of paraffin-embedded rat heart using JMJD7 antibody (CAB7408) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse lung using JMJD7 antibody (CAB7408) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse kidney using JMJD7 antibody (CAB7408) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of A549 cells using JMJD7 antibody (CAB7408). Blue: DAPI for nuclear staining. |
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