Epigenetics & Nuclear Signaling Antibodies 4
Anti-IWS1 Antibody (CAB7375)
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- SKU:
- CAB7375
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-IWS1 Antibody |
| Antibody SKU: | CAB7375 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-819 of human IWS1 (NP_060439.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | NCI-H460, Mouse lung, Mouse spleen, Mouse testis, Mouse ovary, Rat brain |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 570-819 of human IWS1 (NP_060439.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | AAEE DRQL NNQK KPAL KKLT LLPA VVMH LKKQ DLKE TFID SGVM SAIK EWLS PLPD RSLP ALKI REEL LKIL QELP SVSQ ETLK HSGI GRAV MYLY KHPK ESRS NKDM AGKL INEW SRPI FGLT SNYK GMTR EERE QRDL EQMP QRRR MNST GGQT PRRD LEKV LTGE EKAL RPGD PGFC ARAR VPMP SNKD YVVR PKWN VEME SSRF QATS KKGI SRLD KQMR KFTD IRKK SRSA HAVK ISIE GNKM PL |
| Gene ID: | 55677 |
| Uniprot: | Q96ST2 |
| Cellular Location: | Nucleus |
| Calculated MW: | 56kDa/69kDa/91kDa |
| Observed MW: | 92kDa |
| Synonyms: | IWS1 |
| Background: |
| UniProt Protein Function: | IWS1: a Pol II transcription elongation factor essential for mammalian cell proliferation. Interacts with Spt6 and Spt5. Depletion of endogenous human IWS1 results in mRNA processing defects. Three alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:Transcription factor Chromosomal Location of Human Ortholog: 2q14.3 Cellular Component: nucleoplasm; cytoplasm; nucleus Molecular Function:protein binding; DNA binding Biological Process: mRNA transport; regulation of transcription, DNA-dependent; transcription, DNA-dependent; RNA splicing; mRNA processing; regulation of mRNA processing |
| UniProt Code: | Q96ST2 |
| NCBI GenInfo Identifier: | 85542184 |
| NCBI Gene ID: | 55677 |
| NCBI Accession: | Q96ST2.2 |
| UniProt Secondary Accession: | Q96ST2,Q2TB65, Q6P157, Q8N3E8, Q96MI7, Q9NV97, Q9NWH8 |
| UniProt Related Accession: | Q96ST2 |
| Molecular Weight: | 819 |
| NCBI Full Name: | Protein IWS1 homolog |
| NCBI Synonym Full Names: | IWS1 homolog (S. cerevisiae) |
| NCBI Official Symbol: | IWS1 |
| NCBI Protein Information: | protein IWS1 homolog; IWS1-like protein; interacts with Spt6 |
| UniProt Protein Name: | Protein IWS1 homolog |
| UniProt Synonym Protein Names: | IWS1-like protein |
| Protein Family: | IWS1-like protein |
| UniProt Gene Name: | IWS1 |
| UniProt Entry Name: | IWS1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IWS1 antibody (CAB7375) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 5s. |
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