Developmental Biology

Anti-ITSN2 Antibody (CAB16525)

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SKU:
CAB16525
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-ITSN2 Antibody
Antibody SKU:CAB16525
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:U-87MG, LO2, Jurkat, HeLa
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 500-620 of human ITSN2 (NP_006268.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:GKHQ QISG RLQD VRLK KQTQ KTEL EVLD KQCD LEIM EIKQ LQQE LQEY QNKL IYLV PEKQ LLNE RIKN MQFS NTPD SGVS LLHK KSLE KEEL CQRL KEQL DALE KETA SKLS EMDS FNNQ L
Gene ID:50618
Uniprot:Q9NZM3
Cellular Location:Cytoplasm
Calculated MW:135kDa/141kDa/190kDa/193kDa
Observed MW:180kDa
Synonyms:ITSN2, PRO2015, SH3D1B, SH3P18, SWA, SWAP
Background:This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis.
UniProt Protein Function:Adapter protein that may provide indirect link between the endocytic membrane traffic and the actin assembly machinery. May regulate the formation of clathrin-coated vesicles (CCPs). Seems to be involved in CCPs maturation including invagination or budding. Involved in endocytosis of integrin beta-1 (ITGB1) and transferrin receptor (TFR). Plays a role in dendrite formation by melanocytes (PubMed:23999003).
NCBI Summary:This gene encodes a cytoplasmic protein which contains SH3 domains. This protein is a member of a family of proteins involved in clathrin-mediated endocytosis. Intersectin 2 is thought to regulate the formation of clathrin-coated vesicles and also may function in the induction of T cell antigen receptor (TCR) endocytosis. [provided by RefSeq, Jan 2017]
UniProt Code:Q9NZM3
NCBI GenInfo Identifier:194294521
NCBI Gene ID:50618
NCBI Accession:NP_006268.2
UniProt Secondary Accession:Q9NZM3,O95062, Q15812, Q9HAK4, Q9NXE6, Q9NYG0, Q9NZM2 Q9ULG4,
UniProt Related Accession:Q9NZM3
Molecular Weight:193 kDa
NCBI Full Name:intersectin-2 isoform 1
NCBI Synonym Full Names:intersectin 2
NCBI Official Symbol:ITSN2  
NCBI Official Synonym Symbols:SWA; SWAP; SH3D1B; SH3P18; PRO2015  
NCBI Protein Information:intersectin-2
UniProt Protein Name:Intersectin-2
UniProt Synonym Protein Names:SH3 domain-containing protein 1B; SH3P18; SH3P18-like WASP-associated protein
Protein Family:Intersectin
UniProt Gene Name:ITSN2  
Anti-ITSN2 Antibody (CAB16525)Western blot analysis of extracts of various cell lines, using ITSN2 antibody (CAB16525) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
Anti-ITSN2 Antibody (CAB16525)Immunofluorescence analysis of C6 cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ITSN2 Antibody (CAB16525)Immunofluorescence analysis of L929 cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-ITSN2 Antibody (CAB16525)Immunofluorescence analysis of U-2 OS cells using ITSN2 antibody (CAB16525) at dilution of 1:100. Blue: DAPI for nuclear staining.
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