Developmental Biology
Anti-ISL1 Antibody (CAB6383)
- SKU:
- CAB6383
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Developmental Biology
Description
| Antibody Name: | Anti-ISL1 Antibody |
| Antibody SKU: | CAB6383 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human ISL1 (NP_002193.2). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HeLa, SH-SY5Y, Mouse thymus, Rat thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-349 of human ISL1 (NP_002193.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MGDM GDPP KKKR LISL CVGC GNQI HDQY ILRV SPDL EWHA ACLK CAEC NQYL DESC TCFV RDGK TYCK RDYI RLYG IKCA KCSI GFSK NDFV MRAR SKVY HIEC FRCV ACSR QLIP GDEF ALRE DGLF CRAD HDVV ERAS LGAG DPLS PLHP ARPL QMAA EPIS ARQP ALRP HVHK QPEK TTRV RTVL NEKQ LHTL RTCY AANP RPDA LMKE QLVE MTGL SPRV IRVW FQNK RCKD KKRS IMMK QLQQ QQPN DKTN IQGM TGTP MVAA SPER HDGG LQAN PVEV QSYQ PPWK VLSD FALQ SDID QPAF QQLV NFSE GGPG SNST GSEV ASMS SQLP DTPN SMVA SPIE A |
| Gene ID: | 3670 |
| Uniprot: | P61371 |
| Cellular Location: | Nucleus |
| Calculated MW: | 39kDa |
| Observed MW: | 50kDa |
| Synonyms: | ISL1, ISLET1, Isl-1 |
| Background: | This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. |
| UniProt Protein Function: | ISL1: Binds to one of the cis-acting domain of the insulin gene enhancer. |
| UniProt Protein Details: | Protein type:Cell development/differentiation; Nuclear receptor co-regulator; DNA-binding; Transcription factor; Motility/polarity/chemotaxis Chromosomal Location of Human Ortholog: 5q11.1 Cellular Component: nucleoplasm; mitochondrion; cytoplasm; nucleus Molecular Function:ligand-dependent nuclear receptor binding; zinc ion binding; bHLH transcription factor binding; estrogen receptor binding; chromatin binding Biological Process: positive regulation of granulocyte macrophage colony-stimulating factor production; positive regulation of interleukin-12 production; positive regulation of interleukin-1 alpha production; negative regulation of transcription from RNA polymerase II promoter; positive regulation of tyrosine phosphorylation of Stat3 protein; negative regulation of estrogen receptor signaling pathway; pancreas development; positive regulation of cell proliferation; ventricular cardiac muscle morphogenesis; neuron fate specification; negative regulation of neuron apoptosis; sensory system development; neural crest cell migration; positive regulation of DNA binding; trigeminal nerve development; pharyngeal system development; axon regeneration; spinal cord motor neuron cell fate specification; positive regulation of insulin secretion; positive regulation of interleukin-6 production; spinal cord motor neuron differentiation; positive regulation of tumor necrosis factor production; positive regulation of interleukin-1 beta production; positive regulation of interferon-gamma production; negative regulation of neuron differentiation; positive regulation of angiogenesis; mesenchymal cell differentiation; visceral motor neuron differentiation; pituitary gland development; negative regulation of inflammatory response; positive regulation of transcription from RNA polymerase II promoter; peripheral nervous system neuron axonogenesis; retinal ganglion cell axon guidance |
| NCBI Summary: | This gene encodes a member of the LIM/homeodomain family of transcription factors. The encoded protein binds to the enhancer region of the insulin gene, among others, and may play an important role in regulating insulin gene expression. The encoded protein is central to the development of pancreatic cell lineages and may also be required for motor neuron generation. Mutations in this gene have been associated with maturity-onset diabetes of the young. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P61371 |
| NCBI GenInfo Identifier: | 47606423 |
| NCBI Gene ID: | 3670 |
| NCBI Accession: | P61371.1 |
| UniProt Secondary Accession: | P61371,P20663, P47894, |
| UniProt Related Accession: | P61371 |
| Molecular Weight: | 349 |
| NCBI Full Name: | Insulin gene enhancer protein ISL-1 |
| NCBI Synonym Full Names: | ISL LIM homeobox 1 |
| NCBI Official Symbol: | ISL1 |
| NCBI Official Synonym Symbols: | Isl-1; ISLET1 |
| NCBI Protein Information: | insulin gene enhancer protein ISL-1; islet-1; ISL1 transcription factor, LIM/homeodomain |
| UniProt Protein Name: | Insulin gene enhancer protein ISL-1 |
| Protein Family: | Insulin gene enhancer protein |
| UniProt Gene Name: | ISL1 |
| UniProt Entry Name: | ISL1_HUMAN |
 | Western blot analysis of extracts of various cell lines, using ISL1 antibody (CAB6383) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
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