Name: Anti-IRF6 Antibody (CAB6246)
Brand: Developmental Biology
SKU: CAB6246
Availability: OutOfStock

Antibody Name:	Anti-IRF6 Antibody
Antibody SKU:	CAB6246
Antibody Size:	20uL, 50uL, 100uL
Application:	WB
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 115-300 of human IRF6 (NP_006138.1).

Application:	WB
Recommended Dilution:	WB 1:500 - 1:2000
Reactivity:	Human, Mouse, Rat
Positive Samples:	22Rv1, BT-474, HT-29, MCF7, 293T, Mouse liver, Mouse kidney, Rat liver

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 115-300 of human IRF6 (NP_006138.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	DIPQ PQGS IINP GSTG SAPW DEKD NDVD EEDE EDEL DQSQ HHVP IQDT FPFL NING SPMA PASV GNCS VGNC SPEA VWPK TEPL EMEV PQAP IQPF YSSP ELWI SSLP MTDL DIKF QYRG KEYG QTMT VSNP QGCR LFYG DLGP MPDQ EELF GPVS LEQV KFPG PEHI TNEK QKLF TSKL LDVM DR
Gene ID:	3664
Uniprot:	O14896
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	41kDa/53kDa
Observed MW:	53kDa

Synonyms:

IRF6, LPS, OFC6, PIT, PPS, PPS1, VWS, VWS1

Background:

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	IRF6: Probable DNA-binding transcriptional activator. Key determinant of the keratinocyte proliferation-differentiation switch involved in appropriate epidermal development. Plays a role in regulating mammary epithelial cell proliferation. May regulate WDR65 transcription. Interacts with SERPINB5. Expressed in normal mammary epithelial cells. Expression is reduced or absent in breast carcinomas. Belongs to the IRF family.
UniProt Protein Details:	Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 1q32.3-q41 Cellular Component: cytoplasm; cytosol; nucleus Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: keratinocyte differentiation; negative regulation of cell proliferation; transcription, DNA-dependent; positive regulation of transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; keratinocyte proliferation; cell cycle arrest; cell development Disease: Orofacial Cleft 6, Susceptibility To; Popliteal Pterygium Syndrome; Van Der Woude Syndrome 1
NCBI Summary:	This gene encodes a member of the interferon regulatory transcription factor (IRF) family. Family members share a highly-conserved N-terminal helix-turn-helix DNA-binding domain and a less conserved C-terminal protein-binding domain. The encoded protein may be a transcriptional activator. Mutations in this gene can cause van der Woude syndrome and popliteal pterygium syndrome. Mutations in this gene are also associated with non-syndromic orofacial cleft type 6. Alternate splicing results in multiple transcript variants.[provided by RefSeq, May 2011]
UniProt Code:	O14896
NCBI GenInfo Identifier:	3122293
NCBI Gene ID:	3664
NCBI Accession:	O14896.1
UniProt Secondary Accession:	O14896,B4DLE2, D3DT90, F5GWX8, G0ZTL0,
UniProt Related Accession:	O14896
Molecular Weight:	467
NCBI Full Name:	Interferon regulatory factor 6
NCBI Synonym Full Names:	interferon regulatory factor 6
NCBI Official Symbol:	IRF6
NCBI Official Synonym Symbols:	LPS; PIT; PPS; VWS; OFC6; PPS1; VWS1
NCBI Protein Information:	interferon regulatory factor 6
UniProt Protein Name:	Interferon regulatory factor 6
Protein Family:	Interferon regulatory factor
UniProt Gene Name:	IRF6
UniProt Entry Name:	IRF6_HUMAN

Western blot analysis of extracts of various cell lines, using IRF6 antibody (CAB6246) at 1:1000 dilution._Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution._Lysates/proteins: 25ug per lane._Blocking buffer: 3% nonfat dry milk in TBST._Detection: ECL Enhanced Kit (RM00021)._Exposure time: 15s.

ELISA Kits

Human IRF6 (Interferon regulatory factor 6) ELISA Kit (HUFI07764)

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)