Epigenetics & Nuclear Signaling Antibodies 1

Anti-IRF4 Antibody (CAB0524)

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SKU:
CAB0524
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling

Description

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Antibody Name:Anti-IRF4 Antibody
Antibody SKU:CAB0524
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 150-350 of human IRF4 (NP_002451.2).
Application:WB
Recommended Dilution:WB 1:500 - 1:2000
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse spleen, Mouse lung, Rat spleen, Rat lung, Rat thymus
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 150-350 of human IRF4 (NP_002451.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:HPYT MTTP YPSL PAQQ VHNY MMPP LDRS WRDY VPDQ PHPE IPYQ CPMT FGPR GHHW QGPA CENG CQVT GTFY ACAP PESQ APGV PTEP SIRS AEAL AFSD CRLH ICLY YREI LVKE LTTS SPEG CRIS HGHT YDAS NLDQ VLFP YPED NGQR KNIE KLLS HLER GVVL WMAP DGLY AKRL CQSR IYWD GPLA LCND RPNK L
Gene ID:3662
Uniprot:Q15306
Cellular Location:Nucleus
Calculated MW:51kDa
Observed MW:52kDa
Synonyms:IRF4, LSIRF, MUM1, NF-EM5, SHEP8, IRF-4
Background:The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene.
UniProt Protein Function:IRF4: Transcriptional activator. Binds to the interferon- stimulated response element (ISRE) of the MHC class I promoter. Binds the immunoglobulin lambda light chain enhancer, together with PU.1. Probably plays a role in ISRE-targeted signal transduction mechanisms specific to lymphoid cells. Interacts with SPIB and DEF6. Not induced by interferons. Lymphoid cells. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:DNA-binding; Transcription factor; Oncoprotein

Chromosomal Location of Human Ortholog: 6p25-p23

Cellular Component: nucleoplasm; membrane; cytoplasm; cytosol; nucleus

Molecular Function:protein binding; sequence-specific DNA binding; protein-lysine N-methyltransferase activity; transcription factor binding; transcription factor activity

Biological Process: transcription, DNA-dependent; T cell activation; cytokine and chemokine mediated signaling pathway; positive regulation of interleukin-2 biosynthetic process; positive regulation of transcription, DNA-dependent; regulation of T-helper cell differentiation; myeloid dendritic cell differentiation; defense response to protozoan; peptidyl-lysine methylation; positive regulation of interleukin-10 biosynthetic process; positive regulation of interleukin-4 biosynthetic process; positive regulation of interleukin-13 biosynthetic process; negative regulation of toll-like receptor signaling pathway; positive regulation of transcription from RNA polymerase II promoter; positive regulation of DNA binding

Disease: Skin/hair/eye Pigmentation, Variation In, 8

NCBI Summary:The protein encoded by this gene belongs to the IRF (interferon regulatory factor) family of transcription factors, characterized by an unique tryptophan pentad repeat DNA-binding domain. The IRFs are important in the regulation of interferons in response to infection by virus, and in the regulation of interferon-inducible genes. This family member is lymphocyte specific and negatively regulates Toll-like-receptor (TLR) signaling that is central to the activation of innate and adaptive immune systems. A chromosomal translocation involving this gene and the IgH locus, t(6;14)(p25;q32), may be a cause of multiple myeloma. Alternatively spliced transcript variants have been found for this gene. [provided by RefSeq, Aug 2010]
UniProt Code:Q15306
NCBI GenInfo Identifier:2497445
NCBI Gene ID:3662
NCBI Accession:Q15306.1
UniProt Secondary Accession:Q15306,Q5VUI7, Q99660,
UniProt Related Accession:Q15306
Molecular Weight:451
NCBI Full Name:Interferon regulatory factor 4
NCBI Synonym Full Names:interferon regulatory factor 4
NCBI Official Symbol:IRF4  
NCBI Official Synonym Symbols:MUM1; LSIRF; SHEP8; NF-EM5  
NCBI Protein Information:interferon regulatory factor 4; multiple myeloma oncogene 1; lymphocyte-specific interferon regulatory factor
UniProt Protein Name:Interferon regulatory factor 4
UniProt Synonym Protein Names:Lymphocyte-specific interferon regulatory factor; LSIRF; Multiple myeloma oncogene 1; NF-EM5
Protein Family:Interferon regulatory factor
UniProt Gene Name:IRF4  
UniProt Entry Name:IRF4_HUMAN
Anti-IRF4 Antibody (CAB0524)Western blot analysis of extracts of various cell lines, using IRF4 antibody (CAB0524) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
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