Epigenetics & Nuclear Signaling Antibodies 3
Anti-IRF2 Antibody (CAB2558)
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- SKU:
- CAB2558
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-IRF2 Antibody |
| Antibody SKU: | CAB2558 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SW480, NIH/3T3 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 120-349 of human IRF2 (NP_002190.2). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | KKGK KPKT EKED KVKH IKQE PVES SLGL SNGV SDLS PEYA VLTS TIKN EVDS TVNI IVVG QSHL DSNI ENQE IVTN PPDI CQVV EVTT ESDE QPVS MSEL YPLQ ISPV SSYA ESET TDSV PSDE ESAE GRPH WRKR NIEG KQYL SNMG TRGS YLLP GMAS FVTS NKPD LQVT IKEE SNPV PYNS SWPP FQDL PLSS SMTP ASSS SRPD RETR ASVI KKTS DITQ ARVK SC |
| Gene ID: | 3660 |
| Uniprot: | P14316 |
| Cellular Location: | Nucleus |
| Calculated MW: | 39kDa |
| Observed MW: | 42kDa |
| Synonyms: | IRF2, IRF-2 |
| Background: | IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. |
| UniProt Protein Function: | IRF2: Specifically binds to the upstream regulatory region of type I IFN and IFN-inducible MHC class I genes (the interferon consensus sequence (ICS)) and represses those genes. Also acts as an activator for several genes including H4 and IL7. Constitutively binds to the ISRE promoter to activate IL7. Involved in cell cycle regulation through binding the site II (HiNF-M) promoter region of H4 and activating transcription during cell growth. Antagonizes IRF1 transcriptional activation. Interacts with BRD7, IRF2BP1 and IRF2BP2. Interacts with CREBBP in growing cells; the interaction acetylates IRF2 and regulates IRF2-dependent H4 promoter activity. By viruses and IFN. Expressed throughout the epithelium of the colon. Also expressed in lamina propria. Belongs to the IRF family. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:Transcription factor; DNA-binding Chromosomal Location of Human Ortholog: 4q34.1-q35.1 Cellular Component: nucleoplasm; focal adhesion; cytoplasm; cytosol Molecular Function:protein binding; DNA binding; transcription factor activity Biological Process: cell proliferation; regulation of transcription, DNA-dependent; transcription, DNA-dependent; cytokine and chemokine mediated signaling pathway; positive regulation of transcription from RNA polymerase II promoter; negative regulation of transcription from RNA polymerase II promoter; blood coagulation |
| NCBI Summary: | IRF2 encodes interferon regulatory factor 2, a member of the interferon regulatory transcription factor (IRF) family. IRF2 competitively inhibits the IRF1-mediated transcriptional activation of interferons alpha and beta, and presumably other genes that employ IRF1 for transcription activation. However, IRF2 also functions as a transcriptional activator of histone H4. [provided by RefSeq, Jul 2008] |
| UniProt Code: | P14316 |
| NCBI GenInfo Identifier: | 20141499 |
| NCBI Gene ID: | 3660 |
| NCBI Accession: | P14316.2 |
| UniProt Secondary Accession: | P14316,Q6IAS7, Q96B99, D6RCK5, H0Y8S3, |
| UniProt Related Accession: | P14316 |
| Molecular Weight: | 39,156 Da |
| NCBI Full Name: | Interferon regulatory factor 2 |
| NCBI Synonym Full Names: | interferon regulatory factor 2 |
| NCBI Official Symbol: | IRF2 |
| NCBI Official Synonym Symbols: | IRF-2 |
| NCBI Protein Information: | interferon regulatory factor 2 |
| UniProt Protein Name: | Interferon regulatory factor 2 |
| Protein Family: | Interferon regulatory factor |
| UniProt Gene Name: | IRF2 |
| UniProt Entry Name: | IRF2_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IRF2 antibody (CAB2558) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. |
 | Immunohistochemistry of paraffin-embedded rat heart using IRF2 antibody (CAB2558) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded rat brain using IRF2 antibody (CAB2558) at dilution of 1:200 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human gastric cancer using IRF2 antibody (CAB2558) at dilution of 1:200 (40x lens). |
 | Immunofluorescence analysis of HeLa cells using IRF2 antibody (CAB2558). Blue: DAPI for nuclear staining. |
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