Antibody Name:	Anti-IQGAP1 Antibody
Antibody SKU:	CAB5679
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	A-549, 293T, HepG2, HT-29, HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 500-750 of human IQGAP1 (NP_003861.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	ELMK LKAQ AHAE NNEF ITWN DIQA CVDH VNLV VQEE HERI LAIG LINE ALDE GDAQ KTLQ ALQI PAAK LEGV LAEV AQHY QDTL IRAK REKA QEIQ DESA VLWL DEIQ GGIW QSNK DTQE AQKF ALGI FAIN EAVE SGDV GKTL SALR SPDV GLYG VIPE CGET YHSD LAEA KKKK LAVG DNNS KWVK HWVK GGYY YYHN LETQ EGGW DEPP NFVQ NSMQ LSRE EIQS SISG VTAA YNRE QLWL ANEG LIT
Gene ID:	8826
Uniprot:	P46940
Cellular Location:	Cell membrane
Calculated MW:	189kDa
Observed MW:	189kDa

Synonyms:

IQGAP1, HUMORFA01, SAR1, p195

Background:

This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines.

UniProt Protein Function:	IQGAP1: Binds to activated CDC42 but does not stimulate its GTPase activity. It associates with calmodulin. Could serve as an assembly scaffold for the organization of a multimolecular complex that would interface incoming signals to the reorganization of the actin cytoskeleton at the plasma membrane. May promote neurite outgrowth. Interacts with CDC42; the interaction is demonstrated with IQGAP1 in GTP-bound and in nucleotide-free state. Interacts with RAC1. Does not interact with RHOA. Interacts with TSG101. Interacts with PAK6. Expressed in the placenta, lung, and kidney. A lower level expression is seen in the heart, liver, skeletal muscle and pancreas.
UniProt Protein Details:	Protein type:Motility/polarity/chemotaxis; GAPs, Ras; GAPs Chromosomal Location of Human Ortholog: 15q26.1 Cellular Component: actin cytoskeleton; actin filament; axon; cytoplasm; cytosol; extrinsic to internal side of plasma membrane; focal adhesion; growth cone; microtubule; microtubule cytoskeleton; midbody; neuron projection; plasma membrane; ruffle Molecular Function:calcium ion binding; calmodulin binding; GTPase inhibitor activity; phosphatidylinositol-3,4,5-triphosphate binding; protein binding; protein domain specific binding; protein kinase binding; protein phosphatase binding; protein serine/threonine kinase activator activity Biological Process: epidermal growth factor receptor signaling pathway; positive regulation of protein kinase activity; signal transduction
NCBI Summary:	This gene encodes a member of the IQGAP family. The protein contains four IQ domains, one calponin homology domain, one Ras-GAP domain and one WW domain. It interacts with components of the cytoskeleton, with cell adhesion molecules, and with several signaling molecules to regulate cell morphology and motility. Expression of the protein is upregulated by gene amplification in two gastric cancer cell lines. [provided by RefSeq, Jul 2008]
UniProt Code:	P46940
NCBI GenInfo Identifier:	1170586
NCBI Gene ID:	8826
NCBI Accession:	P46940.1
UniProt Secondary Accession:	P46940,A7MBM3,
UniProt Related Accession:	P46940
Molecular Weight:	189,252 Da
NCBI Full Name:	Ras GTPase-activating-like protein IQGAP1
NCBI Synonym Full Names:	IQ motif containing GTPase activating protein 1
NCBI Official Symbol:	IQGAP1
NCBI Official Synonym Symbols:	SAR1; p195; HUMORFA01
NCBI Protein Information:	ras GTPase-activating-like protein IQGAP1
UniProt Protein Name:	Ras GTPase-activating-like protein IQGAP1
UniProt Synonym Protein Names:	p195
Protein Family:	Ras GTPase-activating-like protein
UniProt Gene Name:	IQGAP1
UniProt Entry Name:	IQGA1_HUMAN

	Western blot analysis of extracts of various cell lines, using IQGAP1 antibody (CAB5679) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 10s.
	Immunohistochemistry of paraffin-embedded human placenta using IQGAP1 antibody (CAB5679) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded rat spleen using IQGAP1 antibody (CAB5679) at dilution of 1:200 (40x lens).
	Immunohistochemistry of paraffin-embedded mouse lung using IQGAP1 antibody (CAB5679) at dilution of 1:200 (40x lens).
	Immunofluorescence analysis of C6 cells using IQGAP1 Polyclonal Antibody (CAB5679) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.