Antibody Name:	Anti-IP3R Antibody
Antibody SKU:	CAB14008
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1250 of human IP3R (NP_001161744.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Mouse brain, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1100-1250 of human IP3R (NP_001161744.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	TSQD VDNY KQIK QDLD QLRS IVEK SELW VYKG QGPD ETMD GASG ENEH KKTE EGNN KPQK HEST SSYN YRVV KEIL IRLS KLCV QESA SVRK SRKQ QQRL LRNM GAHA VVLE LLQI PYEK AEDT KMQE IMRL AHEF LQNF CAGN QQNQ ALL
Gene ID:	3708
Uniprot:	Q14643
Cellular Location:	Endoplasmic reticulum membrane, Multi-pass membrane protein
Calculated MW:	306-313kDa
Observed MW:	315kDa

Synonyms:

ITPR1, ACV, CLA4, INSP3R1, IP3R, IP3R1, PPP1R94, SCA15, SCA16, SCA29

Background:

This gene encodes an intracellular receptor for inositol 1, 4, 5-trisphosphate. Upon stimulation by inositol 1, 4, 5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene.

UniProt Protein Function:	IP3R1: a multi-pass endoplasmic reticulum membrane receptor for inositol 1,4,5-trisphosphate, a second messenger that mediates the release of intracellular calcium. Plays a role in ER stress-induced apoptosis. Cytoplasmic calcium released from the ER triggers apoptosis by the activation of CaMKII, eventually leading to the activation of downstream apoptosis pathways. Part of a cGMP kinase signaling complex that includes alpha-actin, calponin H1, phospholamban, PKG1 and IP3R1. Widely expressed. The receptor contains a calcium channel in its C-terminal extremity. Its large N-terminal cytoplasmic region has the ligand-binding site in the N-terminus and modulatory sites in the middle portion immediately upstream of the channel region. Phosphorylation by PKA prevents the ligand-induced opening of the calcium channels. Belongs to the ryanodine-inositol 1,4,5-triphosphate receptor ca(2+) channel (rir-cac) family. Eight alternatively spliced isoforms have been described.
UniProt Protein Details:	Protein type:Channel, ligand-gated; Channel, calcium; Membrane protein, multi-pass; Membrane protein, integral Chromosomal Location of Human Ortholog: 3p26.1 Cellular Component: calcineurin complex; endoplasmic reticulum; endoplasmic reticulum membrane; integral to membrane; membrane; nuclear inner membrane; nucleolus; platelet dense granule membrane; postsynaptic density; sarcoplasmic reticulum Molecular Function:calcium channel inhibitor activity; calcium ion transmembrane transporter activity; inositol 1,4,5-triphosphate-sensitive calcium-release channel activity; intracellular ligand-gated calcium channel activity; phosphoinositide binding; protein binding Biological Process: blood coagulation; calcium ion transport; endoplasmic reticulum calcium ion homeostasis; energy reserve metabolic process; epidermal growth factor receptor signaling pathway; epithelial fluid transport; fibroblast growth factor receptor signaling pathway; innate immune response; inositol phosphate-mediated signaling; negative regulation of calcium-mediated signaling; nerve growth factor receptor signaling pathway; phospholipase C activation; platelet activation; post-embryonic development; regulation of insulin secretion; release of sequestered calcium ion into cytosol; response to hypoxia; signal transduction; stimulatory C-type lectin receptor signaling pathway; vascular endothelial growth factor receptor signaling pathway; voluntary musculoskeletal movement
NCBI Summary:	This gene encodes an intracellular receptor for inositol 1,4,5-trisphosphate. Upon stimulation by inositol 1,4,5-trisphosphate, this receptor mediates calcium release from the endoplasmic reticulum. Mutations in this gene cause spinocerebellar ataxia type 15, a disease associated with an heterogeneous group of cerebellar disorders. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Nov 2009]
UniProt Code:	Q14643
NCBI GenInfo Identifier:	519668682
NCBI Gene ID:	3708
NCBI Accession:	Q14643.3
UniProt Secondary Accession:	Q14643,Q14660, Q99897, E7EPX7, E9PDE9,
UniProt Related Accession:	Q14643
Molecular Weight:
NCBI Full Name:	Inositol 1,4,5-trisphosphate receptor type 1
NCBI Synonym Full Names:	inositol 1,4,5-trisphosphate receptor type 1
NCBI Official Symbol:	ITPR1
NCBI Official Synonym Symbols:	ACV; CLA4; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1; PPP1R94
NCBI Protein Information:	inositol 1,4,5-trisphosphate receptor type 1
UniProt Protein Name:	Inositol 1,4,5-trisphosphate receptor type 1
UniProt Synonym Protein Names:	IP3 receptor isoform 1; IP3R 1; InsP3R1; Type 1 inositol 1,4,5-trisphosphate receptor; Type 1 InsP3 receptor
Protein Family:	Inositol 1,4,5-trisphosphate receptor
UniProt Gene Name:	ITPR1
UniProt Entry Name:	ITPR1_HUMAN

	Western blot analysis of extracts of various cell lines, using IP3R antibody (CAB14008) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 10s.
	Immunofluorescence analysis of U2OS cells using IP3R antibody (CAB14008) at dilution of 1:100. Blue: DAPI for nuclear staining.