KO Validated Antibodies 1

[KO Validated] ING5 Rabbit Polyclonal Antibody (CAB7288)

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SKU:
CAB7288
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Epigenetics and Nuclear Signaling
Synonyms:
p28ING5, G5
Research Use:
Epigenetics and Nuclear Signaling
Frequently bought together:

Description

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[KO Validated] ING5 Antibody (CAB7288)

The [KO Validated] ING5 Antibody (CAB7288) is a high-quality antibody developed for reliable detection and analysis of target proteins. This antibody, generated in rabbits, has high reactivity with human samples and has been validated for use in Western blot applications. By binding to the ING5 protein, this antibody enables accurate detection and analysis in various cell types, making it an ideal tool for studies in cancer research and cell biology.ING5 is known to interact with histone deacetylases and histone acetyltransferases, playing a key role in chromatin remodeling and gene transcription regulation.

This antibody is validated for use in WB, IF/ICC, ELISA applications and has demonstrated reactivity against Human samples.

Product Name:[KO Validated] ING5 Antibody
SKU:CAB7288
Size:20μL, 100μL
Reactivity:Human
Conjugate:Unconjugated
Immunogen:Recombinant protein (or fragment).This information is considered to be commercially sensitive.
Sequence:MATA MYLE HYLD SIEN LPCE LQRN FQLM RELD QRTE DKKA EIDI LAAE YIST VKTL SPDQ RVER LQKI QNAY SKCK EYSD DKVQ LAMQ TYEM VDKH IRRL DADL ARFE ADLK DKME GSDF ESSG GRGL KKGR GQKE KRGS RGRG RRTS EEDT PKKK KHKG GSEF TDTI LSVH PSDV LDMP VDPN EPTY CLCH QVSY GEMI GCDN PDCP IEWF HFAC VDLT
Tested Applications:WB IF/ICC ELISA
Recommended Dilution:
WB1:500 - 1:1000
IF/ICC1:50 - 1:100
ELISARecommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Synonyms:p28ING5, G5
Positive Sample:293T
Cellular Localization:Nucleus.
Calculated MW:28kDa
Observed MW:30kDa

This gene encodes a tumor suppressor protein that inhibits cell growth and induces apoptosis. This protein contains a PHD-type zinc finger. It interacts with tumor suppressor p53 and p300, a component of the histone acetyl transferase complex, suggesting a role in transcriptional regulation. Alternative splicing and the use of multiple promoters and 3' terminal exons results in multiple transcript variants.

Purification MethodAffinity purification
Gene ID84289
RRIDAB_2767829
Buffer InformationStore at -20℃. Avoid freeze / thaw cycles. Buffer: PBS containing 50% glycerol, preserved with proclin300 or sodium azide, pH 7.3.
Product ImageWestern blot analysis of lysates from wild type (WT) and ING5 knockout (KO) 293T cells, using [KO Validated] ING5 Rabbit pAb (CAB7288) at 1:1000 dilution. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25μg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (AbGn00020). Exposure time: 1s.
Product ImageImmunofluorescence analysis of MCF-7 cells using [KO Validated] ING5 Rabbit pAb (CAB7288). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (CABS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
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