Cell Biology Antibodies 1

Anti-INF2 Antibody (CAB10038)

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SKU:
CAB10038
Product Type:
Antibody
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Biology

Description

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Antibody Name:Anti-INF2 Antibody
Antibody SKU:CAB10038
Antibody Size:20uL, 50uL, 100uL
Application:WB
Reactivity:Human
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human INF2 (NP_116103.1).
Application:WB
Recommended Dilution:WB 1:500 - 1:1000
Reactivity:Human
Positive Samples:293T, HeLa, BT-474, HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human INF2 (NP_116103.1).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MSVK EGAQ RKWA ALKE KLGP QDSD PTEA NLES ADPE LCIR LLQM PSVV NYSG LRKR LEGS DGGW MVQF LEQS GLDL LLEA LARL SGRG VARI SDAL LQLT CVSC VRAV MNSR QGIE YILS NQGY VRQL SQAL DTSN VMVK KQVF ELLA ALCI YSPE GHVL TLDA LDHY KTVC SQQY RFSI VMNE LSGS DN
Gene ID:64423
Uniprot:Q27J81
Cellular Location:Cytoplasm, perinuclear region
Calculated MW:25kDa/134kDa/135kDa
Observed MW:180kDa
Synonyms:INF2, C14orf151, C14orf173, CMTDIE, FSGS5, pp9484
Background:This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.
UniProt Protein Function:INF2: Severs actin filaments and accelerates their polymerization and depolymerization. Defects in INF2 are the cause of focal segmental glomerulosclerosis type 5 (FSGS5). A renal pathology defined by the presence of segmental sclerosis in glomeruli and resulting in proteinuria, reduced glomerular filtration rate and edema. Renal insufficiency often progresses to end-stage renal disease, a highly morbid state requiring either dialysis therapy or kidney transplantation. Defects in INF2 are the cause of Charcot-Marie-Tooth disease, dominant intermediate type E (CMTDIE). A form of Charcot-Marie-Tooth disease, a disorder of the peripheral nervous system, characterized by progressive weakness and atrophy, initially of the peroneal muscles and later of the distal muscles of the arms. The dominant intermediate type E is characterized by clinical and pathologic features intermediate between demyelinating and axonal peripheral neuropathies, and motor median nerve conduction velocities ranging from 25 to 45 m/sec. Patients additionally manifest focal segmental glomerulonephritis, proteinuria, progression to end- stage renal disease, and a characteristic histologic pattern on renal biopsy. Belongs to the formin homology family. 3 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:

Protein type:Unknown function

Chromosomal Location of Human Ortholog: 14q32.33

Cellular Component: perinuclear region of cytoplasm

Disease: Charcot-marie-tooth Disease, Dominant Intermediate E; Focal Segmental Glomerulosclerosis 5

NCBI Summary:This gene represents a member of the formin family of proteins. It is considered a diaphanous formin due to the presence of a diaphanous inhibitory domain located at the N-terminus of the encoded protein. Studies of a similar mouse protein indicate that the protein encoded by this locus may function in polymerization and depolymerization of actin filaments. Mutations at this locus have been associated with focal segmental glomerulosclerosis 5.[provided by RefSeq, Aug 2010]
UniProt Code:Q27J81
NCBI GenInfo Identifier:166215588
NCBI Gene ID:64423
NCBI Accession:Q27J81.2
UniProt Secondary Accession:Q27J81,Q27J83, Q69YL8, Q6P1X7, Q6PK22, Q86TR7, Q9BRM1 Q9H6N1,
UniProt Related Accession:Q27J81
Molecular Weight:25,954 Da
NCBI Full Name:Inverted formin-2
NCBI Synonym Full Names:inverted formin, FH2 and WH2 domain containing
NCBI Official Symbol:INF2  
NCBI Official Synonym Symbols:FSGS5; CMTDIE; pp9484; C14orf151; C14orf173  
NCBI Protein Information:inverted formin-2
UniProt Protein Name:Inverted formin-2
UniProt Synonym Protein Names:HBEBP2-binding protein C
Protein Family:Inverted formin
UniProt Gene Name:INF2  
UniProt Entry Name:INF2_HUMAN
Anti-INF2 Antibody (CAB10038)Western blot analysis of extracts of various cell lines, using INF2 antibody (CAB10038) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
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