Cell Cycle Antibodies 1
Anti-IKZF1 Antibody (CAB1850)
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- SKU:
- CAB1850
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Research Area:
- Cell Cycle
Description
| Antibody Name: | Anti-IKZF1 Antibody |
| Antibody SKU: | CAB1850 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IKZF1 (NP_001207694.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Raji, Mouse thymus |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 1-270 of human IKZF1 (NP_001207694.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MDAD EGQD MSQV SGKE SPPV SDTP DEGD EPMP IPED LSTT SGGQ QSSK SDRV VASN VKVE TQSD EENG RACE MNGE ECAE DLRM LDAS GEKM NGSH RDQG SSAL SGVG GIRL PNGK LKCD ICGI ICIG PNVL MVHK RSHT GERP FQCN QCGA SFTQ KGNL LRHI KLHS GEKP FKCH LCNY ACRR RDAL TGHL RTHS VIKE ETNH SEMA EDLC KIGS ERSL VLDR LASN VAKR KSSM PQKF LGDK GLSD TPYD SSAS YEKE NEMM KSHV MD |
| Gene ID: | 10320 |
| Uniprot: | Q13422 |
| Cellular Location: | Cytoplasm, Nucleus, Nucleus |
| Calculated MW: | 24-31kDa/41-57kDa |
| Observed MW: | 55-75kDa |
| Synonyms: | IKZF1, CVID13, Hs.54452, IK1, IKAROS, LYF1, LyF-1, PPP1R92, PRO0758, ZNFN1A1 |
| Background: | This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). |
| UniProt Protein Function: | Ikaros: a transcription factor of the ikaros C2H2-type zinc-finger protein family. Binds and activates the enhancer (delta-A element) of the CD3-delta gene. Functions in the specification and the maturation of the T-lymphocyte. Also interacts with a critical control element in the TDT (terminal deoxynucleotidyltransferase) promoter as well as with the promoters for other genes expressed during early stages of B- and T-cell development. Deletions in Ikaros have been observed in a subset of pre-B-cell acute lymphoblastic leukemia (B-ALL) cases. Seven alternatively spliced human isoforms have been described. |
| UniProt Protein Details: | Protein type:C2H2-type zinc finger protein; DNA-binding; Transcription factor Chromosomal Location of Human Ortholog: 7p12.2 Cellular Component: centric heterochromatin; protein complex; cytoplasm; nucleus Molecular Function:DNA binding; sequence-specific DNA binding; protein heterodimerization activity; metal ion binding; transcription factor activity Biological Process: Peyer's patch development; thymus development; transcription, DNA-dependent; negative regulation of transcription from RNA polymerase II promoter; positive regulation of multicellular organism growth; chromatin modification; cell cycle; lymph node development; positive regulation of neutrophil differentiation; retina development in camera-type eye; natural killer cell differentiation; B cell differentiation; forebrain development; positive regulation of NK T cell differentiation; mesoderm development; positive regulation of transcription from RNA polymerase II promoter; T cell differentiation |
| NCBI Summary: | This gene encodes a transcription factor that belongs to the family of zinc-finger DNA-binding proteins associated with chromatin remodeling. The expression of this protein is restricted to the fetal and adult hemo-lymphopoietic system, and it functions as a regulator of lymphocyte differentiation. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. Most isoforms share a common C-terminal domain, which contains two zinc finger motifs that are required for hetero- or homo-dimerization, and for interactions with other proteins. The isoforms, however, differ in the number of N-terminal zinc finger motifs that bind DNA and in nuclear localization signal presence, resulting in members with and without DNA-binding properties. Only a few isoforms contain the requisite three or more N-terminal zinc motifs that confer high affinity binding to a specific core DNA sequence element in the promoters of target genes. The non-DNA-binding isoforms are largely found in the cytoplasm, and are thought to function as dominant-negative factors. Overexpression of some dominant-negative isoforms have been associated with B-cell malignancies, such as acute lymphoblastic leukemia (ALL). [provided by RefSeq, May 2014] |
| UniProt Code: | Q13422 |
| NCBI GenInfo Identifier: | 3913926 |
| NCBI Gene ID: | 10320 |
| NCBI Accession: | Q13422.1 |
| UniProt Related Accession: | Q13422 |
| Molecular Weight: | |
| NCBI Full Name: | DNA-binding protein Ikaros |
| NCBI Synonym Full Names: | IKAROS family zinc finger 1 |
| NCBI Official Symbol: | IKZF1Â Â |
| NCBI Official Synonym Symbols: | IK1; LYF1; LyF-1; CVID13; IKAROS; PPP1R92; PRO0758; ZNFN1A1; Hs.54452Â Â |
| NCBI Protein Information: | DNA-binding protein Ikaros |
| UniProt Protein Name: | DNA-binding protein Ikaros |
| UniProt Synonym Protein Names: | Ikaros family zinc finger protein 1; Lymphoid transcription factor LyF-1 |
| Protein Family: | Ikaros family zinc finger protein |
| UniProt Gene Name: | IKZF1Â Â |
| UniProt Entry Name: | IKZF1_HUMAN |
 | Western blot analysis of extracts of Raji cells, using Ikaros antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s. |
 | Chromatin immunoprecipitation analysis of extracts of K-562 cells, using Ikaros antibody and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input. |
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