Immunology Antibodies 1
Anti-IKKBeta Antibody (CAB0714)
- SKU:
- CAB0714
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Immunology
Description
| Antibody Name: | Anti-IKKBeta Antibody |
| Antibody SKU: | CAB0714 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 517-756 of human IKKBeta (NP_001547.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | Jurkat, HepG2 |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 517-756 of human IKKBeta (NP_001547.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | MEQA VELC GREN EVKL LVER MMAL QTDI VDLQ RSPM GRKQ GGTL DDLE EQAR ELYR RLRE KPRD QRTE GDSQ EMVR LLLQ AIQS FEKK VRVI YTQL SKTV VCKQ KALE LLPK VEEV VSLM NEDE KTVV RLQE KRQK ELWN LLKI ACSK VRGP VSGS PDSM NASR LSQP GQLM SQPS TASN SLPE PAKK SEEL VAEA HNLC TLLE NAIQ DTVR EQDQ SFTA LDWS WLQT EEEE HSCL EQAS |
| Gene ID: | 3551 |
| Uniprot: | O14920 |
| Cellular Location: | Cytoplasm, Membrane raft, Nucleus |
| Calculated MW: | 29kDa/79kDa/85kDa/86kDa |
| Observed MW: | 87kDa |
| Synonyms: | IKBKB, IKK-beta, IKK2, IKKB, IMD15, NFKBIKB, IKK beta |
| Background: | The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. |
| UniProt Protein Function: | IKKB: a kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Preferentially found as a heterodimer with IKK-alpha but also as an homodimer. |
| UniProt Protein Details: | Protein type:Kinase, protein; EC 2.7.11.10; Protein kinase, Ser/Thr (non-receptor); Protein kinase, Other; Other group; IKK family Chromosomal Location of Human Ortholog: 8p11.2 Cellular Component: internal side of plasma membrane; cytoplasm; IkappaB kinase complex; cytosol; nucleus; lipid raft Molecular Function:protein serine/threonine kinase activity; protein binding; protein homodimerization activity; IkappaB kinase activity; protein heterodimerization activity; protein kinase binding; ATP binding; protein kinase activity Biological Process: I-kappaB kinase/NF-kappaB cascade; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; toll-like receptor 3 signaling pathway; T cell receptor signaling pathway; protein amino acid phosphorylation; activation of NF-kappaB transcription factor; toll-like receptor 10 signaling pathway; toll-like receptor 5 signaling pathway; B cell homeostasis; positive regulation of interferon type I production; inflammatory response; toll-like receptor 4 signaling pathway; positive regulation of I-kappaB kinase/NF-kappaB cascade; MyD88-independent toll-like receptor signaling pathway; response to virus; toll-like receptor 2 signaling pathway; MyD88-dependent toll-like receptor signaling pathway; serine phosphorylation of STAT protein; toll-like receptor signaling pathway; innate immune response; positive regulation of transcription from RNA polymerase II promoter; toll-like receptor 9 signaling pathway; I-kappaB phosphorylation; negative regulation of apoptosis Disease: Immunodeficiency 15 |
| NCBI Summary: | The protein encoded by this gene phosphorylates the inhibitor in the inhibitor/NF-kappa-B complex, causing dissociation of the inhibitor and activation of NF-kappa-B. The encoded protein itself is found in a complex of proteins. Several transcript variants, some protein-coding and some not, have been found for this gene. [provided by RefSeq, Sep 2011] |
| UniProt Code: | O14920 |
| NCBI GenInfo Identifier: | 14285497 |
| NCBI Gene ID: | 3551 |
| NCBI Accession: | O14920.1 |
| UniProt Secondary Accession: | O14920,O75327, B4DZ30, B4E0U4, |
| UniProt Related Accession: | O14920 |
| Molecular Weight: | 86,564 Da |
| NCBI Full Name: | Inhibitor of nuclear factor kappa-B kinase subunit beta |
| NCBI Synonym Full Names: | inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase beta |
| NCBI Official Symbol: | IKBKB |
| NCBI Official Synonym Symbols: | IKK2; IKKB; IMD15; NFKBIKB; IKK-beta |
| NCBI Protein Information: | inhibitor of nuclear factor kappa-B kinase subunit beta; IKK-B; I-kappa-B kinase 2; I-kappa-B-kinase beta; nuclear factor NF-kappa-B inhibitor kinase beta |
| UniProt Protein Name: | Inhibitor of nuclear factor kappa-B kinase subunit beta |
| UniProt Synonym Protein Names: | I-kappa-B kinase 2; IKK2; Nuclear factor NF-kappa-B inhibitor kinase beta |
| UniProt Gene Name: | IKBKB |
| UniProt Entry Name: | IKKB_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IKKb antibody (CAB0714) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 5s. |
 | Immunofluorescence analysis of U2OS cells using IKKb antibody (CAB0714) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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