Signal Transduction Antibodies 1

Anti-IGF2R Antibody (CAB13022)

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SKU:
CAB13022
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Signal Transduction

Description

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Antibody Name:Anti-IGF2R Antibody
Antibody SKU:CAB13022
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2).
Application:WB IHC IF
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:HepG2
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 2327-2491 of human IGF2R (NP_000867.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:YKKE RRET VISK LTTC CRRS SNVS YKYS KVNK EEET DENE TEWL MEEI QLPP PRQG KEGQ ENGH ITTK SVKA LSSL HGDD QDSE DEVL TIPE VKVH SGRG AGAE SSHP VRNA QSNA LQER EDDR VGLV RGEK ARKG KSSS AQQK TVSS TKLV SFHD DSDE DLLH I
Gene ID:3482
Uniprot:P11717
Cellular Location:Lysosome membrane, Single-pass type I membrane protein
Calculated MW:274kDa
Observed MW:274KDa
Synonyms:IGF2R, CD222, CI-M6PR, CIMPR, M6P-R, M6P/IGF2R, MPR 300, MPR1, MPR300, MPRI
Background:This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611).
UniProt Protein Function:IGF2R: a multifunctional type I transmembrane protein receptor that binds insulin-like growth factor 2 at the cell surface and mannose-6-phosphate-tagged proteins in the trans-Golgi network. Also known as the cation-independent mannose-6 phosphate (M6P) receptor. Clears IGF2 from the cell surface to attenuate signaling, and transports M6P-tagged lysosomal enzymes from the Golgi complex and the cell surface to lysosomes. M6P-tagged lysosomal enzymes bind specifically to mannose-6- phosphate receptors in the Golgi apparatus and the resulting receptor-ligand complex is transported to an acidic prelyosomal compartment where the low pH mediates the dissociation of the complex. Acts as a positive regulator of T-cell coactivation, by binding DPP4. Belongs to the MRL1/IGF2R family.
UniProt Protein Details:

Protein type:Membrane protein, integral; Receptor, misc.; Vesicle

Chromosomal Location of Human Ortholog: 6q26

Cellular Component: cell surface; clathrin coat; early endosome; endocytic vesicle; endosome; extracellular space; focal adhesion; integral to plasma membrane; late endosome; lysosomal membrane; membrane; nuclear envelope lumen; perinuclear region of cytoplasm; trans-Golgi network; trans-Golgi network transport vesicle

Molecular Function:enzyme binding; G-protein alpha-subunit binding; G-protein coupled receptor activity; glycoprotein binding; identical protein binding; insulin-like growth factor II binding; insulin-like growth factor receptor activity; mannose binding; phosphoprotein binding; protein binding; receptor activity; retinoic acid binding; transporter activity

Biological Process: G-protein coupled receptor protein signaling pathway; insulin-like growth factor receptor signaling pathway; liver development; organ regeneration; positive regulation of apoptosis; post-embryonic development; receptor-mediated endocytosis; response to retinoic acid; signal transduction; spermatogenesis

Disease: Hepatocellular Carcinoma

NCBI Summary:This gene encodes a receptor for both insulin-like growth factor 2 and mannose 6-phosphate. The binding sites for each ligand are located on different segments of the protein. This receptor has various functions, including in the intracellular trafficking of lysosomal enzymes, the activation of transforming growth factor beta, and the degradation of insulin-like growth factor 2. Mutation or loss of heterozygosity of this gene has been association with risk of hepatocellular carcinoma. The orthologous mouse gene is imprinted and shows exclusive expression from the maternal allele; however, imprinting of the human gene may be polymorphic, as only a minority of individuals showed biased expression from the maternal allele (PMID:8267611). [provided by RefSeq, Nov 2015]
UniProt Code:P11717
NCBI GenInfo Identifier:317373416
NCBI Gene ID:3482
NCBI Accession:P11717.3
UniProt Secondary Accession:P11717,Q7Z7G9, Q96PT5,
UniProt Related Accession:P11717
Molecular Weight:274,375 Da
NCBI Full Name:Cation-independent mannose-6-phosphate receptor
NCBI Synonym Full Names:insulin like growth factor 2 receptor
NCBI Official Symbol:IGF2R  
NCBI Official Synonym Symbols:MPR1; MPRI; CD222; CIMPR; M6P-R; MPR300; CI-M6PR; MPR 300; M6P/IGF2R  
NCBI Protein Information:cation-independent mannose-6-phosphate receptor
UniProt Protein Name:Cation-independent mannose-6-phosphate receptor
UniProt Synonym Protein Names:300 kDa mannose 6-phosphate receptor; MPR 300; Insulin-like growth factor 2 receptor; Insulin-like growth factor II receptor; IGF-II receptor; M6P/IGF2 receptor; M6P/IGF2R; CD_antigen: CD222
Protein Family:Cation-independent mannose-6-phosphate receptor
UniProt Gene Name:IGF2R  
UniProt Entry Name:MPRI_HUMAN
Anti-IGF2R Antibody (CAB13022)Western blot analysis of extracts of HepG2 cells, using IGF2R antibody (CAB13022) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-IGF2R Antibody (CAB13022)Immunohistochemistry of paraffin-embedded human placenta using IGF2R antibody (CAB13022) at dilution of 1:100 (40x lens).
Anti-IGF2R Antibody (CAB13022)Immunohistochemistry of paraffin-embedded human gastric cancer using IGF2R antibody (CAB13022) at dilution of 1:100 (40x lens).
Anti-IGF2R Antibody (CAB13022)Immunofluorescence analysis of C6 cells using IGF2R antibody (CAB13022) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-IGF2R Antibody (CAB13022)Immunofluorescence analysis of HeLa cells using IGF2R antibody (CAB13022) at dilution of 1:100. Blue: DAPI for nuclear staining.
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