Name: Anti-IGF2BP2 Antibody (CAB2749)
Brand: Epigenetics & Nuclear Signaling Antibodies 3
SKU: CAB2749
Availability: OutOfStock

Antibody Name:	Anti-IGF2BP2 Antibody
Antibody SKU:	CAB2749
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human IGF2BP2 (NP_006539.3).

Application:	WB IHC IF
Recommended Dilution:	WB 1:200 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse
Positive Samples:	Jurkat, Mouse ovary

Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human IGF2BP2 (NP_006539.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	NFFN PKEE VKLE AHIR VPSS TAGR VIGK GGKT VNEL QNLT SAEV IVPR DQTP DENE EVIV RIIG HFFA SQTA QRKI REIV QQVK QQEQ KYPQ GVAS QRSK
Gene ID:	10644
Uniprot:	Q9Y6M1
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	54kDa/58kDa/59kDa/61kDa/66kDa
Observed MW:	66kDa

Synonyms:	IGF2BP2, IMP-2, IMP2, VICKZ2
Background:	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms.

UniProt Protein Function:	IGF2BP2: Binds to the 5'-UTR of the insulin-like growth factor 2 (IGF2) mRNAs. Binding is isoform-specific. May regulate translation of target mRNAs. Belongs to the RRM IMP/VICKZ family. 6 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Protein type:RNA-binding; Translation Chromosomal Location of Human Ortholog: 3q27.2 Cellular Component: cytoskeleton; cytoplasm; nucleus; cytosol Molecular Function:protein binding; mRNA 3'-UTR binding; nucleotide binding; translation regulator activity; mRNA 5'-UTR binding Biological Process: anatomical structure morphogenesis; mRNA transport; negative regulation of translation; gene expression; regulation of cytokine biosynthetic process Disease: Diabetes Mellitus, Noninsulin-dependent
NCBI Summary:	This gene encodes a member of the IGF-II mRNA-binding protein (IMP) family. The protein encoded by this gene contains four KH domains and two RRM domains. It functions by binding to the 5' UTR of the insulin-like growth factor 2 (IGF2) mRNA and regulating IGF2 translation. Alternative promoter usage and alternate splicing result in multiple variants encoding different isoforms. [provided by RefSeq, May 2014]
UniProt Code:	Q9Y6M1
NCBI GenInfo Identifier:	224471831
NCBI Gene ID:	10644
NCBI Accession:	Q9Y6M1.2
UniProt Secondary Accession:	Q9Y6M1,A0A4Z0, B3FTN2, B3FTN3, B3FTN4,
UniProt Related Accession:	Q9Y6M1
Molecular Weight:	599
NCBI Full Name:	Insulin-like growth factor 2 mRNA-binding protein 2
NCBI Synonym Full Names:	insulin-like growth factor 2 mRNA binding protein 2
NCBI Official Symbol:	IGF2BP2
NCBI Official Synonym Symbols:	IMP2; IMP-2; VICKZ2
NCBI Protein Information:	insulin-like growth factor 2 mRNA-binding protein 2; VICKZ family member 2; IGF2 mRNA-binding protein 2; IGF-II mRNA-binding protein 2
UniProt Protein Name:	Insulin-like growth factor 2 mRNA-binding protein 2
UniProt Synonym Protein Names:	Hepatocellular carcinoma autoantigen p62; IGF-II mRNA-binding protein 2; VICKZ family member 2
Protein Family:	Insulin-like growth factor 2 mRNA-binding protein
UniProt Gene Name:	IGF2BP2
UniProt Entry Name:	IF2B2_HUMAN

	Western blot analysis of extracts of various cell lines, using IGF2BP2 antibody (CAB2749) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 30s.
	Immunohistochemistry of paraffin-embedded mouse liver using IGF2BP2 antibody (CAB2749) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of U2OS cells using IGF2BP2 antibody (CAB2749) at dilution of 1:100. Blue: DAPI for nuclear staining.