Cell Death Antibodies 2
Anti-IFT57 Antibody (CAB7191)
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- SKU:
- CAB7191
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Death
Description
| Antibody Name: | Anti-IFT57 Antibody |
| Antibody SKU: | CAB7191 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-429 of human IFT57 (NP_060480.1). |
| Application: | WB |
| Recommended Dilution: | WB 1:500 - 1:2000 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | HL-60, HT-29, MCF7, U-937, Mouse kidney, Mouse testis, Mouse brain, Rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 170-429 of human IFT57 (NP_060480.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | VEEL EEES VAED DAEL TLNK VDEE FVEE ETDN EENF IDLN VLKA QTYH LDMN ETAK QEDI LEST TDAA EWSL EVER VLPQ LKVT IRTD NKDW RIHV DQMH QHRS GIES ALKE TKGF LDKL HNEI TRTL EKIS SREK YINN QLEN LVQE YRAA QAQL SEAK ERYQ QGNG GVTE RTRL LSEV MEEL EKVK QEME EKGS SMTD GAPL VKIK QSLT KLKQ ETVE MDIR IGIV EHTL LQSK LKEK SNMT RNMH ATVI PEPA TGFY |
| Gene ID: | 55081 |
| Uniprot: | Q9NWB7 |
| Cellular Location: | Cytoplasm, cilium basal body, cytoskeleton |
| Calculated MW: | 49kDa |
| Observed MW: | 49kDa |
| Synonyms: | IFT57, ESRRBL1, HIPPI, MHS4R2 |
| Background: |
| UniProt Protein Function: | IFT57: Required for the formation of cilia. Plays an indirect role in sonic hedgehog signaling, cilia being required for all activity of the hedgehog pathway. Has pro- apoptotic function via its interaction with HIP1, leading to recruit caspase-8 (CASP8) and trigger apoptosis. Has the ability to bind DNA sequence motif 5'-AAAGACATG-3' present in the promoter of caspase genes such as CASP1, CASP8 and CASP10, suggesting that it may act as a transcription regulator; however the relevance of such function remains unclear. Belongs to the IFT57 family. |
| UniProt Protein Details: | Chromosomal Location of Human Ortholog: 3q13.13 Cellular Component: Golgi apparatus; centrosome; photoreceptor connecting cilium; cilium Molecular Function:protein binding; DNA binding Biological Process: regulation of apoptosis; caspase activation; smoothened signaling pathway; regulation of transcription, DNA-dependent; transcription, DNA-dependent; apoptosis; organelle organization and biogenesis; neural tube closure; intraflagellar transport; cilium biogenesis; heart looping; negative regulation of epithelial cell proliferation |
| UniProt Code: | Q9NWB7 |
| NCBI GenInfo Identifier: | 74734638 |
| NCBI Gene ID: | 55081 |
| NCBI Accession: | Q9NWB7.1 |
| UniProt Secondary Accession: | Q9NWB7,Q96DA9, |
| UniProt Related Accession: | Q9NWB7 |
| Molecular Weight: | 429 |
| NCBI Full Name: | Intraflagellar transport protein 57 homolog |
| NCBI Synonym Full Names: | intraflagellar transport 57 |
| NCBI Official Symbol: | IFT57 |
| NCBI Official Synonym Symbols: | HIPPI; MHS4R2; ESRRBL1 |
| NCBI Protein Information: | intraflagellar transport protein 57 homolog; HIP1 protein interactor; HIP1-interacting protein; dermal papilla-derived protein 8; intraflagellar transport 57 homolog; estrogen-related receptor beta like 1; estrogen-related receptor beta-like protein 1; huntingtin interacting protein-1 interacting protein |
| UniProt Protein Name: | Intraflagellar transport protein 57 homolog |
| UniProt Synonym Protein Names: | Dermal papilla-derived protein 8; Estrogen-related receptor beta-like protein 1; HIP1-interacting protein; MHS4R2 |
| Protein Family: | Intraflagellar transport protein |
| UniProt Gene Name: | IFT57 |
| UniProt Entry Name: | IFT57_HUMAN |
 | Western blot analysis of extracts of various cell lines, using IFT57 antibody (CAB7191) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s. |
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