Antibody Name:	Anti-IFRD1 Antibody
Antibody SKU:	CAB7439
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human IFRD1 (NP_001541.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:100 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	SW480, BT-474, HeLa, Raji, Mouse lung, Mouse skeletal muscle, Mouse spinal cord, Rat brain

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human IFRD1 (NP_001541.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MPKN KKRN TPHR GSSA GGGG SGAA AATA ATAG GQHR NVQP FSDE DASI ETMS HCSG YSDP SSFA EDGP EVLD EEGT QEDL EYKL KGLI DLTL DKSA KTRQ AALE GIKN ALAS KMLY EFIL ERRM TLTD SIER CLKK GKSD EQRA AAAL ASVL CIQL GPGI ESEE ILKT LGPI LKKI ICDG SASM QARQ TCAT CFGV CCFI ATDD ITEL YSTL ECLE NIFT KSYL KEKD TTVI CSTP NTVL
Gene ID:	3475
Uniprot:	O00458
Cellular Location:
Calculated MW:	45kDa/50kDa
Observed MW:	45kDa, 50kDa

Synonyms:

IFRD1, PC4, TIS7

Background:

This gene is an immediate early gene that encodes a protein related to interferon-gamma. This protein may function as a transcriptional co-activator/repressor that controls the growth and differentiation of specific cell types during embryonic development and tissue regeneration. Mutations in this gene are associated with sensory/motor neuropathy with ataxia. This gene may also be involved in modulating the pathogenesis of cystic fibrosis lung disease. Alternate splicing results in multiple transcript variants.

UniProt Protein Function:	IFRD1: Could play a role in regulating gene activity in the proliferative and/or differentiative pathways induced by NGF. May be an autocrine factor that attenuates or amplifies the initial ligand-induced signal. Belongs to the IFRD family. 2 isoforms of the human protein are produced by alternative splicing.
UniProt Protein Details:	Chromosomal Location of Human Ortholog: 7q31.1 Cellular Component: nucleus Biological Process: multicellular organismal development; adult somatic muscle development; myoblast cell fate determination
NCBI Summary:	This gene is an immediate early gene that encodes a protein related to interferon-gamma. This protein may function as a transcriptional co-activator/repressor that controls the growth and differentiation of specific cell types during embryonic development and tissue regeneration. Mutations in this gene are associated with sensory/motor neuropathy with ataxia. This gene may also be involved in modulating the pathogenesis of cystic fibrosis lung disease. Alternate splicing results in multiple transcript variants. [provided by RefSeq, Oct 2010]
UniProt Code:	O00458
NCBI GenInfo Identifier:	68846516
NCBI Gene ID:	3475
NCBI Accession:	O00458.4
UniProt Secondary Accession:	O00458,O75234, Q5U013, Q9BVE4, B7Z5G1,
UniProt Related Accession:	O00458
Molecular Weight:	45,336 Da
NCBI Full Name:	Interferon-related developmental regulator 1
NCBI Synonym Full Names:	interferon-related developmental regulator 1
NCBI Official Symbol:	IFRD1
NCBI Official Synonym Symbols:	PC4; TIS7
NCBI Protein Information:	interferon-related developmental regulator 1; TPA induced sequence 7; pheochromocytoma cell-4; nerve growth factor-inducible protein PC4; 12-O-tetradecanoylphorbol-13-acetate-induced sequence 7
UniProt Protein Name:	Interferon-related developmental regulator 1
UniProt Synonym Protein Names:	Nerve growth factor-inducible protein PC4
Protein Family:	Interferon-related developmental regulator
UniProt Gene Name:	IFRD1
UniProt Entry Name:	IFRD1_HUMAN

	Western blot analysis of extracts of various cell lines, using IFRD1 antibody (CAB7439) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Immunohistochemistry of paraffin-embedded mouse lung using IFRD1 antibody (CAB7439) at dilution of 1:100 (40x lens).