Name: Anti-IFITM3 Antibody (CAB13070)[KO Validated]
Brand: KO Validated Antibodies 1
SKU: CAB13070
Availability: OutOfStock

Antibody Name:	Anti-IFITM3 Antibody [KO Validated]
Antibody SKU:	CAB13070
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).

Application:	WB IHC IF
Recommended Dilution:	WB 1:500 - 1:2000 IHC 1:50 - 1:200 IF 1:50 - 1:100
Reactivity:	Human, Mouse, Rat
Positive Samples:	HeLa

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-133 of human IFITM3 (NP_066362.2).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MNHT VQTF FSPV NSGQ PPNY EMLK EEHE VAVL GAPH NPAP PTST VIHI RSET SVPD HVVW SLFN TLFM NPCC LGFI AFAY SVKS RDRK MVGD VTGA QAYA STAK CLNI WALI LGIL MTIL LIVI PVLI FQAY G
Gene ID:	10410
Uniprot:	Q01628
Cellular Location:	Cell membrane, Late endosome membrane, Lysosome membrane, Single-pass type II membrane protein
Calculated MW:	14kDa
Observed MW:	15kDa

Synonyms:	IFITM3, 1-8U, DSPA2b, IP15
Background:	The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined.

UniProt Protein Function:	IFITM3: IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, namely influenza A H1N1 virus, West Nile virus (WNV), and dengue virus (WNV), by inhibiting the early step(s) of replication. Interacts with SPP1; the interaction reduces OPN expression. By IFN-alpha and IFNG/IFN-gamma. Belongs to the CD225/Dispanin family.
UniProt Protein Details:	Protein type:Membrane protein, integral; Cell surface Chromosomal Location of Human Ortholog: 11p15.5 Cellular Component: lysosomal membrane; late endosome membrane; integral to membrane; plasma membrane Molecular Function:protein binding Biological Process: negative regulation of viral transcription; negative regulation of viral genome replication; negative regulation of virion penetration into host cell; response to virus; cytokine and chemokine mediated signaling pathway; immune response; defense response to virus Disease: Influenza, Severe, Susceptibility To
NCBI Summary:	The protein encoded by this gene is an interferon-induced membrane protein that helps confer immunity to influenza A H1N1 virus, West Nile virus, and dengue virus. Two transcript variants, only one of them protein-coding, have been found for this gene. Another variant encoding an N-terminally truncated isoform has been reported, but the full-length nature of this variant has not been determined. [provided by RefSeq, May 2012]
UniProt Code:	Q01628
NCBI GenInfo Identifier:	20178301
NCBI Gene ID:	10410
NCBI Accession:	Q01628.2
UniProt Secondary Accession:	Q01628,Q53Y76, Q96HK8, Q96J15,
UniProt Related Accession:	Q01628
Molecular Weight:	133
NCBI Full Name:	Interferon-induced transmembrane protein 3
NCBI Synonym Full Names:	interferon induced transmembrane protein 3
NCBI Official Symbol:	IFITM3
NCBI Official Synonym Symbols:	1-8U; IP15; DSPA2b
NCBI Protein Information:	interferon-induced transmembrane protein 3; dispanin subfamily A member 2b; interferon-inducible protein 1-8U
UniProt Protein Name:	Interferon-induced transmembrane protein 3
UniProt Synonym Protein Names:	Dispanin subfamily A member 2b; DSPA2b; Interferon-inducible protein 1-8U
UniProt Gene Name:	IFITM3
UniProt Entry Name:	IFM3_HUMAN

	Western blot analysis of extracts of HeLa cells, using IFITM3 antibody (CAB13070) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
	Western blot analysis of extracts from normal (control) and IFITM3 knockout (KO) HeLa cells, using IFITM3 antibody (CAB13070) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.
	Immunohistochemistry of paraffin-embedded Human colon carcinoma using [KO Validated] IFITM3 Rabbit pAb (CAB13070) at dilution of 1:100 (40x lens).
	Immunofluorescence analysis of C6 cells using [KO] IFITM3 antibody (CAB13070) at dilution of 1:100. Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of L929 cells using [KO] IFITM3 antibody (CAB13070) at dilution of 1:100. Blue: DAPI for nuclear staining.

Antibodies	ELISA Kits
IFITM3 Antibody (PACO06575)	Human Fragilis / IFITM3 ELISA Kit
IFITM3 Antibody (PACO09877)	Human Interferon-induced transmembrane protein 3 (IFITM3) ELISA Kit

Secondary Antibody

Anti-HRP Goat Anti-Rabbit IgG (H+L) Antibody (CABS014)