Cell Death Antibodies 1

Anti-IFI16 Antibody (CAB14002)

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SKU:
CAB14002
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Cell Death

Description

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Antibody Name:Anti-IFI16 Antibody
Antibody SKU:CAB14002
Antibody Size:20uL, 50uL, 100uL
Application:WB IHC
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).
Application:WB IHC
Recommended Dilution:WB 1:500 - 1:2000 IHC 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:Mouse spleen, Mouse lung
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human IFI16 (NP_005522.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:MGKK YKNI VLLK GLEV INDY HFRM VKSL LSND LKLN LKMR EEYD KIQI ADLM EEKF RGDA GLGK LIKI FEDI PTLE DLAE TLKK EKLK VKGP ALSR KRKK EVDA TSPA PSTS STVK TEGA EATP GAQK RKKS TKEK AGPK GSKV SEEQ TQPP SPAG AGMS TAMG RSPS PKTS LSAP PNSS STEN PKTV AKCQ VTPR RNVL QKRP VIVK VLST TKPF EYET PEME KKIM FHAT VATQ TQFF HVKV LNTS LKEK FNGK KIII ISDY LEYD SLLE VNEE STVS EAGP NQTF EVPN KIIN RAKE
Gene ID:3428
Uniprot:Q16666
Cellular Location:Cytoplasm, Nucleus
Calculated MW:75kDa/82kDa/88kDa
Observed MW:88kDa
Synonyms:IFI16, IFNGIP1, PYHIN2
Background:This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
UniProt Protein Function:IFI16: a nuclear protein that may function as a transcriptional repressor. Strongly induced by gamma interferon and, to a lesser extent, by alpha interferon. Binds double-stranded DNA and cell cycle regulatory factors including p53 and Rb. Loss of IFI16 activates p53 checkpoint through NBS1-DNAPK pathway. Inhibits cell growth in the Ras/Raf signaling pathway. May be involved in the senescence of prostate epithelial cells. Expressed in peripheral blood leukocytes, fibroblasts and lymphoid cells. Present in myeloid precursors (CD34+) and throughout monocyte development, but its expression is down-regulated in erythroid and polymorphonuclear precursor cells. Present in prostate, ovary and breast. Four alternatively spliced isoforms have been described. Isoforms-1, -2 and -3 can homo- and hetero-dimerize.
UniProt Protein Details:

Protein type:Transcription factor

Chromosomal Location of Human Ortholog: 1q22

Cellular Component: nucleoplasm; membrane; cytoplasm; nucleolus; nuclear speck; cytosol; nucleus

Molecular Function:identical protein binding; protein binding; double-stranded DNA binding; transcription factor binding

Biological Process: positive regulation of cytokine production; transcription, DNA-dependent; monocyte differentiation; negative regulation of transcription from RNA polymerase II promoter; regulation of gene expression, epigenetic; negative regulation of DNA binding; cellular response to glucose starvation; positive regulation of interleukin-1 beta production; cell proliferation; negative regulation of viral genome replication; negative regulation of innate immune response; positive regulation of interferon type I production; autophagy; innate immune response; hemopoiesis; positive regulation of transcription from RNA polymerase II promoter; myeloid cell differentiation; regulation of autophagy; negative regulation of transcription, DNA-dependent; inflammatory response; defense response to virus; activation of innate immune response; DNA damage response, signal transduction by p53 class mediator resulting in induction of apoptosis

NCBI Summary:This gene encodes a member of the HIN-200 (hematopoietic interferon-inducible nuclear antigens with 200 amino acid repeats) family of cytokines. The encoded protein contains domains involved in DNA binding, transcriptional regulation, and protein-protein interactions. The protein localizes to the nucleoplasm and nucleoli, and interacts with p53 and retinoblastoma-1. It modulates p53 function, and inhibits cell growth in the Ras/Raf signaling pathway. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Apr 2011]
UniProt Code:Q16666
NCBI GenInfo Identifier:118572657
NCBI Gene ID:3428
NCBI Accession:Q16666.3
UniProt Secondary Accession:Q16666,Q59GX0, Q5T3W7, Q5T3W8, Q5T3X0, Q5T3X1, Q5T3X2 Q8N9E5, Q8NEQ7, Q96AJ5, B4DJT8, H3BLV7,
UniProt Related Accession:Q16666
Molecular Weight:785
NCBI Full Name:Gamma-interferon-inducible protein 16
NCBI Synonym Full Names:interferon, gamma-inducible protein 16
NCBI Official Symbol:IFI16  
NCBI Official Synonym Symbols:PYHIN2; IFNGIP1  
NCBI Protein Information:gamma-interferon-inducible protein 16; interferon-gamma induced protein IFI 16; interferon-inducible myeloid differentiation transcriptional activator
UniProt Protein Name:Gamma-interferon-inducible protein 16
UniProt Synonym Protein Names:Interferon-inducible myeloid differentiation transcriptional activator
Protein Family:Gamma-interferon-inducible protein
UniProt Gene Name:IFI16  
UniProt Entry Name:IF16_HUMAN
Anti-IFI16 Antibody (CAB14002)Western blot analysis of extracts of various cell lines, using IFI16 antibody (CAB14002) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 90s.
Anti-IFI16 Antibody (CAB14002)Immunohistochemistry of paraffin-embedded rat liver using IFI16 Antibody (CAB14002) at dilution of 1:100 (40x lens).
Anti-IFI16 Antibody (CAB14002)Immunohistochemistry of paraffin-embedded human kidney cancer using IFI16 Antibody (CAB14002) at dilution of 1:100 (40x lens).
Anti-IFI16 Antibody (CAB14002)Immunohistochemistry of paraffin-embedded mouse lung using IFI16 Antibody (CAB14002) at dilution of 1:100 (40x lens).
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