Antibody Name:	Anti-HUS1 Antibody
Antibody SKU:	CAB5407
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF IP
Reactivity:	Human, Mouse
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).

Application:	WB IF IP
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:100 IP 1:50 - 1:200
Reactivity:	Human, Mouse
Positive Samples:	OVCAR-3, K-562, 293T

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human HUS1 (NP_004498.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MKFR AKIV DGAC LNHF TRIS NMIA KLAK TCTL RISP DKLN FILC DKLA NGGV SMWC ELEQ ENFF NEFQ MEGV SAEN NEIY LELT SENL SRAL KTAQ NARA LKIK LTNK HFPC LTVS VELL SMSS SSRI VTHD IPIK VIPR KLWK DLQE PVVP DPDV SIYL PVLK TMKS VVEK MKNI SNHL VIEA NLDG ELNL KIET ELVC VTTH FKDL GNPP LASE STHE DRNV EHMA EVHI DIRK LLQF LAGQ QVNP TKAL CNIV NNKM VHFD LLHE DVSL QYFI PALS
Gene ID:	3364
Uniprot:	O60921
Cellular Location:	Cytoplasm, Nucleus
Calculated MW:	29kDa/31kDa
Observed MW:	35kDa

Synonyms:

HUS1, hHUS1

Background:

The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants.

UniProt Protein Function:	HUS1: Component of the 9-1-1 cell-cycle checkpoint response complex that plays a major role in DNA repair. The 9-1-1 complex is recruited to DNA lesion upon damage by the RAD17-replication factor C (RFC) clamp loader complex. Acts then as a sliding clamp platform on DNA for several proteins involved in long-patch base excision repair (LP-BER). The 9-1-1 complex stimulates DNA polymerase beta (POLB) activity by increasing its affinity for the 3'-OH end of the primer-template and stabilizes POLB to those sites where LP-BER proceeds; endonuclease FEN1 cleavage activity on substrates with double, nick, or gap flaps of distinct sequences and lengths; and DNA ligase I (LIG1) on long-patch base excision repair substrates. The 9-1-1 complex is necessary for the recruitment of RHNO1 to sites of double-stranded breaks (DSB) occurring during the S phase. Belongs to the HUS1 family.
UniProt Protein Details:	Protein type:DNA repair, damage; Cell cycle regulation Chromosomal Location of Human Ortholog: 7p13-p12 Cellular Component: nucleoplasm; cytoplasm; nucleolus; nucleus Molecular Function:protein binding Biological Process: regulation of protein amino acid phosphorylation; embryonic development; negative regulation of DNA replication; mitotic cell cycle checkpoint; DNA damage checkpoint; DNA repair; DNA replication; response to DNA damage stimulus; protein amino acid phosphorylation; double-strand break repair via homologous recombination; response to UV
NCBI Summary:	The protein encoded by this gene is a component of an evolutionarily conserved, genotoxin-activated checkpoint complex that is involved in the cell cycle arrest in response to DNA damage. This protein forms a heterotrimeric complex with checkpoint proteins RAD9 and RAD1. In response to DNA damage, the trimeric complex interacts with another protein complex consisting of checkpoint protein RAD17 and four small subunits of the replication factor C (RFC), which loads the combined complex onto the chromatin. The DNA damage induced chromatin binding has been shown to depend on the activation of the checkpoint kinase ATM, and is thought to be an early checkpoint signaling event. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2011]
UniProt Code:	O60921
NCBI GenInfo Identifier:	74735462
NCBI Gene ID:	3364
NCBI Accession:	O60921.1
UniProt Secondary Accession:	O60921,B4DFI9,
UniProt Related Accession:	O60921
Molecular Weight:	280
NCBI Full Name:	Checkpoint protein HUS1
NCBI Synonym Full Names:	HUS1 checkpoint homolog (S. pombe)
NCBI Official Symbol:	HUS1
NCBI Official Synonym Symbols:	hHUS1
NCBI Protein Information:	checkpoint protein HUS1; hus1+-like protein
UniProt Protein Name:	Checkpoint protein HUS1
Protein Family:	Checkpoint protein
UniProt Gene Name:	HUS1
UniProt Entry Name:	HUS1_HUMAN

	Western blot analysis of extracts of various cell lines, using HUS1 antibody (CAB5407) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 180s.
	Immunofluorescence analysis of U2OS cells using HUS1 antibody (CAB5407). Blue: DAPI for nuclear staining.
	Immunofluorescence analysis of U2OS cells using HUS1 antibody (CAB5407). Blue: DAPI for nuclear staining.
	Immunoprecipitation analysis of 200ug extracts of 293T cells using 1ug HUS1 antibody (CAB5407). Western blot was performed from the immunoprecipitate using HUS1 antibody (CAB5407) at a dilition of 1:1000.