Product Name:	Anti-Human HLA-A, B, C (MHC Class I) In Vivo Antibody - Ultra Low Endotoxin
Product Code:	IVMB0015
Size:	1mg, 5mg, 25mg, 50mg, 100mg
Clone:	W6/32
Protein:	HLA-A,B,C
Product Type:	Monoclonal Antibody
Synonyms:	Major Histocompatibility Class I, MHC class I, human leukocyte antigen (HLA)
Isotype:	Mouse IgG2a κ
Reactivity:	Baboon, Chimpanzee, Cynomolgus Monkey, Feline, Bovine, Human
Immunogen:	Human tonsil cell membrane
Applications:	B, FC, IHC FF, IP, PhenoCycler®, WB
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

Product Code	Product Name	Endotoxin Level
IVMB0014	Anti-Human HLA-A, B, C (MHC Class I) In Vivo Antibody - Low Endotoxin	Low
IVMB0015	Anti-Human HLA-A, B, C (MHC Class I) In Vivo Antibody - Ultra Low Endotoxin	Ultra Low

Applications:	B, CODEX®, FC, IHC FF, IP, WB
Recommended Usage:	FC The suggested concentration for this HLA-A,B,C Clone W6/32 antibody for staining cells in flow cytometry is ≤ 2.0 µg per 10⁶ in a volume of 100 µl or 100µl of whole blood. Titration of the reagent is recommended for optimal performance for each application. WB The suggested concentration for for use in western blotting is 1-10 µg/ml. WB Bit1 antibody can be used for the detection of Bit1 by Western blot at 1 - 4 µg/mL.
Reactivity:	Baboon, Chimpanzee, Cynomolgus Monkey, Feline, Bovine, Human

Host Species:	Mouse
Specificity:	Clone W6/32 recognizes the human MHC class I molecules HLA-A, -B, and -C.
Antigen Distribution:	HLA-A, -B, and -C are ubiquitously expressed on nucleated cells.
Immunogen:	Human tonsil cell membrane
Concentration:	≥ 5.0 mg/ml
Endotoxin Level:	≤ 0.5 EU/mg as determined by the LAL method
Purity:	≥98% monomer by analytical SEC >95% by SDS Page
Formulation:	This monoclonal antibody is aseptically packaged and formulated in 0.01 M phosphate buffered saline (150 mM NaCl) PBS pH 7.2 - 7.4 with no carrier protein, potassium, calcium or preservatives added.
Preparation:	Functional grade preclinical antibodies are manufactured in an animal free facility using only In vitro protein free cell culture techniques and are purified by a multi-step process including the use of protein A or G to assure extremely low levels of endotoxins, leachable protein A or aggregates.
Storage and Handling:	This antibody is stable for at least one week when stored at 2-8°C. For long term storage, aliquot in working volumes without diluting and store at – 20°C in a manual defrost freezer. Avoid Repeated Freeze Thaw Cycles.

HLA antibody, clone W6/32, recognizes the major histocompatibility complex (MHC) class I molecules human leukocyte antigen (HLA)-A, HLA-B, and HLA-C. MHC class I is ubiquitously expressed on the cell surface of nucleated cells and consists of a 45-kDa type I transmembrane glycoprotein (α-chain or heavy chain) and a 12-kDa soluble protein (β2-microglobulin, β2M)1,2. The α-chain consists of three domains (α1, α2, and α3)3. α1 and α2 form the closed antigen-binding groove and bind to 8-10 aa peptides derived from cytosolic antigens4-6. β2M noncovalently associates with α3, which is essential for MHC stability. MHC class I plays a critical role in the adaptive immune response by presenting endogenous antigens to cytotoxic CD8 T cells. MHC class I molecules can also present exogenous antigens to CD8 T cells via a process known as cross-presentation7. The T cell receptor (TCR)/CD3 complex of CD8 T cells interacts with peptide-MHC class I, which induces CD8 T cell activation and subsequent cell-killing. CD8 molecules also bind to MHC class I, which helps augment TCR signaling8. In contrast to CD8 T cells, MHC class I is an inhibitory ligand for natural killer (NK) cells, promoting self tolerance9. MHC class I also contributes to the positive selection of CD8 T cells and NK cell specificity10,11.