Developmental Biology

Anti-HSPA2 Antibody (CAB7902)

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SKU:
CAB7902
Product Type:
Antibody
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
Antibody Type:
Polyclonal Antibody
Research Area:
Developmental Biology

Description

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Antibody Name:Anti-HSPA2 Antibody
Antibody SKU:CAB7902
Antibody Size:20uL, 50uL, 100uL
Application:WB IF
Reactivity:Human, Mouse, Rat
Host Species:Rabbit
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-639 of human HSPA2 (NP_068814.2).
Application:WB IF
Recommended Dilution:WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:Human, Mouse, Rat
Positive Samples:MCF7, A-549, SKOV3, HeLa, A-431, Mouse brain, Mouse testis, Rat brain, Rat kidney
Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 400-639 of human HSPA2 (NP_068814.2).
Purification Method:Affinity purification
Storage Buffer:Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:IgG
Sequence:TPLS LGIE TAGG VMTP LIKR NTTI PTKQ TQTF TTYS DNQS SVLV QVYE GERA MTKD NNLL GKFD LTGI PPAP RGVP QIEV TFDI DANG ILNV TAAD KSTG KENK ITIT NDKG RLSK DDID RMVQ EAER YKSE DEAN RDRV AAKN ALES YTYN IKQT VEDE KLRG KISE QDKN KILD KCQE VINW LDRN QMAE KDEY EHKQ KELE RVCN PIIS KLYQ GGPG GGSG GGGS GASG GPTI EEVD
Gene ID:3306
Uniprot:P54652
Cellular Location:
Calculated MW:70kDa
Observed MW:75kDa
Synonyms:HSPA2, HSP70-2, HSP70-3
Background:
UniProt Protein Function:HSPA2: In cooperation with other chaperones, Hsp70s stabilize preexistent proteins against aggregation and mediate the folding of newly translated polypeptides in the cytosol as well as within organelles. These chaperones participate in all these processes through their ability to recognize nonnative conformations of other proteins. They bind extended peptide segments with a net hydrophobic character exposed by polypeptides during translation and membrane translocation, or following stress-induced damage. Interacts with ZNF541. Component of the CatSper complex. Belongs to the heat shock protein 70 family.
UniProt Protein Details:

Protein type:Heat shock protein; Chaperone

Chromosomal Location of Human Ortholog: 14q24.1

Cellular Component: male germ cell nucleus; cell surface; mitochondrion; membrane; synaptonemal complex; nucleus; cytosol

Molecular Function:protein binding; enzyme binding; unfolded protein binding; glycolipid binding; ATP binding

Biological Process: G2/M-specific positive regulation of cyclin-dependent protein kinase activity; male meiosis I; protein refolding; male meiosis; response to unfolded protein; spermatid development

UniProt Code:P54652
NCBI GenInfo Identifier:1708307
NCBI Gene ID:3306
NCBI Accession:P54652.1
UniProt Secondary Accession:P54652,Q15508, Q53XM3, Q9UE78,
UniProt Related Accession:P54652
Molecular Weight:70,021 Da
NCBI Full Name:Heat shock-related 70 kDa protein 2
NCBI Synonym Full Names:heat shock 70kDa protein 2
NCBI Official Symbol:HSPA2  
NCBI Official Synonym Symbols:HSP70-2; HSP70-3  
NCBI Protein Information:heat shock-related 70 kDa protein 2; heat shock 70kD protein 2
UniProt Protein Name:Heat shock-related 70 kDa protein 2
UniProt Gene Name:HSPA2  
UniProt Entry Name:HSP72_HUMAN
Anti-HSPA2 Antibody (CAB7902)Western blot analysis of extracts of various cell lines, using HSPA2 antibody (CAB7902) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s.
Anti-HSPA2 Antibody (CAB7902)Immunofluorescence analysis of L929 cells using HSPA2 antibody (CAB7902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Anti-HSPA2 Antibody (CAB7902)Immunofluorescence analysis of U2OS cells using HSPA2 antibody (CAB7902) at dilution of 1:100. Blue: DAPI for nuclear staining.
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