Anti-HSP60 Antibody [LK1]

(No reviews yet) Write a Review
SKU:
STMA00007
Antibody Type:
Monoclonal
Applications:
WB
Applications:
IHC
Applications:
IP
Applications:
ELISA
Applications:
FCM
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Reactivity:
Bovine
Reactivity:
Dog
Reactivity:
Chicken
Reactivity:
Fruit Fly (Drosophila melanogaster)
Reactivity:
Guinea Pig (Cavia porcellus)
Reactivity:
Hamster
Reactivity:
Monkey
Reactivity:
Pig
Reactivity:
Rabbit
Reactivity:
Sheep
Reactivity:
African clawed frog (Xenopus laevis)
Reactivity:
Plant
Reactivity:
Soybean
Reactivity:
Silkworm (Bombyx mori)
Host Species:
Mouse
Isotype:
IgG1
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (STMA00007). Tissue: HaCaT cells. Species: Human. Fixation: Cold 100% methanol at -20°C for 10 minutes. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00007) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.
  • Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (STMA00007). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00007) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT.
  • Western Blot analysis of Human Cell line lysates showing detection of Hsp60 protein using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (STMA00007). Load: 15 µg. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00007) at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.
  • Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-1 (STMA00007). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00007) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.
  • Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK1, (STMA00007). Tissue: skin Fibroblasts. Species: Human. Fixation: Cold 100% methanol for 30 minutes at -20°C. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00007) at 1:1000 for 1 hour at RT. Secondary Antibody: DAKO LSAB2 streptavidin-peroxidase system. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain.
Frequently bought together:

Description

HSP60 Antibody [LK1]

HSP60 Antibody [LK1] is a mouse monoclonal antibody directed against HSP60, offered by Assay Genie for research applications. Reported applications include WB, IHC, IP, ELISA and FCM. Reported reactivity: Human, Mouse, Rat, Bovine, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis), Plant, Soybean and Silkworm (Bombyx mori). Supplied as IgG1, purified by Protein G.

HSP60, also known as Cpn60 or GroEL in prokaryotes, is a highly conserved molecular chaperone essential for protein folding and cellular homeostasis. Present in both prokaryotic and eukaryotic cells, HSP60 prevents protein misfolding and aggregation during biogenesis and under stress conditions. In mammals, HSP60 is localized to the mitochondria, where it partners with its co-chaperonin HSP10 to facilitate the proper folding and assembly of mitochondrial proteins. Structurally, HSP60 forms homo-oligomeric complexes of 7 or 14 subunits, exhibiting ATPase activity and reversible dissociation in the presence of Mg²⁺ and ATP. Its evolutionary conservation is underscored by the ability of human HSP60-HSP10 to functionally replace the bacterial GroEL-GroES system in engineered E. coli strains. Beyond its canonical role in mitochondrial proteostasis, HSP60 has been implicated in immune regulation and cellular stress responses. Elevated levels of HSP60 have been associated with several chronic diseases, including autoimmune disorders, coronary artery disease, diabetes, and neurodegenerative conditions such as Alzheimer’s disease and multiple sclerosis. In neuroscience, HSP60’s role in maintaining mitochondrial integrity is particularly significant, as mitochondrial dysfunction is a central feature of many neurodegenerative diseases. Its dual function in protein quality control and cellular protection positions HSP60 as a promising biomarker and therapeutic target in neurodegeneration research. This antibody is also available conjugated to ATTO 390, ATTO 488, ATTO 594, APC, Biotin, FITC, HRP, PerCP and RPE. Supplied in 200 µg. For research use only; not for diagnostic or therapeutic procedures.

Product Name:HSP60 Antibody [LK1]
Product SKU:STMA00007
Size:200 µg
Target:HSP60
Host Species:Mouse
Antibody Type:Monoclonal
Clone ID:LK1
Research Areas:Cancer, Heat Shock, Cell Signaling, Protein Trafficking, Chaperone Proteins, Tags and Cell Markers, Organelle Markers
Buffer:PBS, 50% glycerol, 0.09% sodium azide
Storage & Shipping:Store at -20°C. Shipped on Blue Ice or 4°C. Conjugated variants should be stored according to the product label.
Format:Liquid
Applications:WB, IHC, IP, ELISA, FCM
Antibody Isotype:IgG1
Purification:Protein G Purified
Concentration:1 mg/ml
Reactivity:Human, Mouse, Rat, Bovine, Dog, Chicken, Fruit Fly (Drosophila melanogaster), Guinea Pig (Cavia porcellus), Hamster, Monkey, Pig, Rabbit, Sheep, African clawed frog (Xenopus laevis), Plant, Soybean, Silkworm (Bombyx mori)
Recommended Dilution:WB (1:20000), IHC (1:100), ICC/IF (1:100), IP (1:200); optimal dilutions for assays should be determined by the user.
Specificity:Detects ~60kDa.
Guarantee:12 months from date of dispatch
Immunogen:Recombinant human HSP60
Immunogen Species:Human
Accession Number:NP_002147.2
Gene ID:3329
Swiss-Prot:P10809
Cellular Localization:Mitochondrion | Mitochondrion Matrix
Alternative Names:HSPD1, HSP60, 60 kDa heat shock protein, mitochondrial, Chaperonin 60, CPN60, HuCHA60, Heat shock protein family D member 1, GroEL homolog, mitochondrial, GROEL, HLD4, HSP 60, HSP65, SPG 13
References:1. Hartl, F.U. (1996) Nature 381: 571-579. 2. Bukau, B. and Horwich, A.L. (1998) Cell 92: 351-366. 3. Hartl, F.U. and Hayer-Hartl, M. (2002) Science 295: 1852- 1858. 4. Jindal, S., et al. (1989) Molecular and Cellular Biology 9: 2279-2283. 5. La Verda, D., et al (1999) Infect Dis. Obstet. Gynecol. 7: 64-71. 6. Itoh, H. et al. (2002) Eur. J. Biochem. 269: 5931-5938. 7. Gupta, S. and Knowlton, A.A. J. Cell Mol Med. 9: 51-58. 8. Deocaris, C.C. et al. (2006) Cell Stress Chaperones 11: 116-128. 9. Lai, H.C. et al. (2007) Am. J. Physiol. Endocrinol. Metab. 292: E292-E297. 10. Gao, Y.L., et al (1995) J. of Immunology 154: 3548-3556. 11. Neuer, A., et al (1997) European Society for Human Reproduction and Embryology 12(5):925-929. 12. Bason, C., et al (2003) Lancet 362(9400): 1971-1977.
Note:For research use only.
View AllClose

0 Reviews

View AllClose

Related Products

Immunocytochemistry/Immunofluorescence analysis using Rabbit Anti-Hsp60 Polyclonal Antibody (STPA00004). Tissue: Heat Shocked Cervical cancer cell line (HeLa). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Rabbit Anti-Hsp60 Polyclonal Antibody (STPA00004) at 1:100 for 12 hours at 4°C. Secondary Antibody: FITC Goat Anti-Rabbit (green) at 1:200 for 2 hours at RT. Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (STPA00004). Tissue: Adrenal. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (STPA00004).

Anti-HSP60 Antibody

HSP60 AntibodyHSP60 Antibody is a rabbit polyclonal antibody directed against HSP60, offered by Assay Genie for research applications. Reported applications include WB, IHC, ICC/IF, IP and ELISA...
Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2 (STMA00008). Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00008) at 1:100000 for 12 hours at 4°C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 µl for 2 minutes at RT. Immunohistochemistry analysis using Mouse Anti-Hsp60 Monoclonal Antibody, Clone LK-2 (STMA00008). Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp60 Monoclonal Antibody (STMA00008) at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Cytoplasmic Staining.

Anti-HSP60 Antibody [LK2]

HSP60 Antibody [LK2]HSP60 Antibody [LK2] is a mouse monoclonal antibody directed against HSP60, offered by Assay Genie for research applications. Reported applications include WB, IHC and FCM...
Immunohistochemistry analysis using Rabbit Anti-HSP60 Polyclonal Antibody (STPA00033). Tissue: Liver. Species: Human. Fixation: Formalin fixed paraffin-embedded. Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (STPA00033). Western blot analysis of Parasite Lysates showing detection of HSP60 protein using Rabbit Anti-HSP60 Polyclonal Antibody (STPA00033). Primary Antibody: Rabbit Anti-HSP60 Polyclonal Antibody (STPA00033) at 1:1000.

Anti-HSP60 (P. falciparum) Antibody

HSP60 (P. falciparum) AntibodyHSP60 (P. falciparum) Antibody is a rabbit polyclonal antibody directed against HSP60 (P. falciparum), offered by Assay Genie for research applications. Reported...