Name: Anti-HSD17B3 Antibody (CAB7687)
Brand: Metabolism Antibodies 2
SKU: CAB7687
Availability: OutOfStock

Antibody Name:	Anti-HSD17B3 Antibody
Antibody SKU:	CAB7687
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IF
Reactivity:	Human, Mouse, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human HSD17B3 (NP_000188.1).

Application:	WB IF
Recommended Dilution:	WB 1:500 - 1:2000 IF 1:50 - 1:200
Reactivity:	Human, Mouse, Rat
Positive Samples:	Rat lung, Rat intestine

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 1-310 of human HSD17B3 (NP_000188.1).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	MGDV LEQF FILT GLLV CLAC LAKC VRFS RCVL LNYW KVLP KSFL RSMG QWAV ITGA GDGI GKAY SFEL AKRG LNVV LISR TLEK LEAI ATEI ERTT GRSV KIIQ ADFT KDDI YEHI KEKL AGLE IGIL VNNV GMLP NLLP SHFL NAPD EIQS LIHC NITS VVKM TQLI LKHM ESRQ KGLI LNIS SGIA LFPW PLYS MYSA SKAF VCAF SKAL QEEY KAKE VIIQ VLTP YAVS TAMT KYLN TNVI TKTA DEFV KESL NYVT IGGE TCGC LAHE ILAG FLSL IPAW AFYS GAFQ RLLL THYV AYLK LNTK VR
Gene ID:	3293
Uniprot:	P37058
Cellular Location:
Calculated MW:	29kDa/34kDa
Observed MW:	34kDa

Synonyms:	HSD17B3, EDH17B3, SDR12C2
Background:	This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia.

UniProt Protein Function:	HSD17B3: Favors the reduction of androstenedione to testosterone. Uses NADPH while the two other EDH17B enzymes use NADH. Defects in HSD17B3 are the cause of male pseudohermaphrodism with gynecomastia (MPH). These individuals have unambiguous female external genitalia at birth, but fail to menstruate at the time of expected puberty and instead virilize as evidenced by growth of the phallus. Breast development may or may not take place. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 17-beta-HSD 3 subfamily.
UniProt Protein Details:	Protein type:EC 1.1.1.64; Oxidoreductase; Lipid Metabolism - androgen and estrogen Chromosomal Location of Human Ortholog: 9q22 Cellular Component: endoplasmic reticulum membrane; intracellular membrane-bound organelle Molecular Function:testosterone 17-beta-dehydrogenase (NADP+) activity Biological Process: steroid metabolic process; male genitalia development; androgen biosynthetic process Disease: 17-beta Hydroxysteroid Dehydrogenase Iii Deficiency
NCBI Summary:	This isoform of 17 beta-hydroxysteroid dehydrogenase is expressed predominantly in the testis and catalyzes the conversion of androstenedione to testosterone. It preferentially uses NADP as cofactor. Deficiency can result in male pseudohermaphroditism with gynecomastia. [provided by RefSeq, Jul 2008]
UniProt Code:	P37058
NCBI GenInfo Identifier:	4557649
NCBI Gene ID:	3293
NCBI Accession:	NP_000188.1
UniProt Related Accession:	P37058
Molecular Weight:	34,516 Da
NCBI Full Name:	testosterone 17-beta-dehydrogenase 3
NCBI Synonym Full Names:	hydroxysteroid (17-beta) dehydrogenase 3
NCBI Official Symbol:	HSD17B3
NCBI Official Synonym Symbols:	EDH17B3; SDR12C2
NCBI Protein Information:	testosterone 17-beta-dehydrogenase 3; 17-beta-HSD3; 17-beta-HSD 3; 17-beta-hydroxysteroid dehydrogenase type 3; testicular 17-beta-hydroxysteroid dehydrogenase; short chain dehydrogenase/reductase family 12C, member 2
UniProt Protein Name:	Testosterone 17-beta-dehydrogenase 3
UniProt Synonym Protein Names:	17-beta-hydroxysteroid dehydrogenase type 3; 17-beta-HSD 3; Testicular 17-beta-hydroxysteroid dehydrogenase
Protein Family:	Testosterone 17-beta-dehydrogenase
UniProt Gene Name:	HSD17B3
UniProt Entry Name:	DHB3_HUMAN

	Western blot analysis of extracts of various cell lines, using HSD17B3 antibody (CAB7687) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 30s.
	Immunofluorescence analysis of U2OS cells using HSD17B3 antibody (CAB7687) at dilution of 1:100. Blue: DAPI for nuclear staining.