Antibody Name:	HSD11B2 Rabbit Polyclonal Antibody
Antibody SKU:	CAB8077
Antibody Size:	20uL, 50uL, 100uL
Application:	WB IHC
Reactivity:	Human, Rat
Host Species:	Rabbit
Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).

Application:	WB IHC
Recommended Dilution:	WB 1:200 - 1:1000 IHC 1:50 - 1:100
Reactivity:	Human, Rat
Positive Samples:	HepG2

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 266-405 of human HSD11B2 (NP_000187.3).
Purification Method:	Affinity purification
Storage Buffer:	Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Isotype:	IgG
Sequence:	KTES VRNV GQWE KRKQ LLLA NLPQ ELLQ AYGK DYIE HLHG QFLH SLRL AMSD LTPV VDAI TDAL LAAR PRRR YYPG QGLG LMYF IHYY LPEG LRRR FLQA FFIS HCLP RALQ PGQP GTTP PQDA AQDP NLSP GPSP AVAR
Gene ID:	3291
Uniprot:	P80365
Cellular Location:	Endoplasmic reticulum, Microsome
Calculated MW:	44kDa
Observed MW:	44kDa

Synonyms:

HSD11B2, AME, AME1, HSD11K, HSD2, SDR9C3

Background:

There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension.

UniProt Protein Function:	HSD11B2: Catalyzes the conversion of cortisol to the inactive metabolite cortisone. Modulates intracellular glucocorticoid levels, thus protecting the nonselective mineralocorticoid receptor from occupation by glucocorticoids. Defects in HSD11B2 are the cause of apparent mineralocorticoid excess (AME). An autosomal recessive form of low-renin hypertension. It is usually diagnosed within the first years of life and is characterized by polyuria and polydipsia, failure to thrive, hypernatremia, severe hypertension with low renin and aldosterone levels, profound hypokalemia with metabolic alkalosis, and most often nephrocalcinosis. Belongs to the short-chain dehydrogenases/reductases (SDR) family.
UniProt Protein Details:	Protein type:Lipid Metabolism - androgen and estrogen; Oxidoreductase; EC 1.1.1.-; Lipid Metabolism - C21-steroid hormone Chromosomal Location of Human Ortholog: 16q22 Cellular Component: endoplasmic reticulum membrane Molecular Function:11-beta-hydroxysteroid dehydrogenase activity Biological Process: glucocorticoid biosynthetic process Disease: Apparent Mineralocorticoid Excess
NCBI Summary:	There are at least two isozymes of the corticosteroid 11-beta-dehydrogenase, a microsomal enzyme complex responsible for the interconversion of cortisol and cortisone. The type I isozyme has both 11-beta-dehydrogenase (cortisol to cortisone) and 11-oxoreductase (cortisone to cortisol) activities. The type II isozyme, encoded by this gene, has only 11-beta-dehydrogenase activity. In aldosterone-selective epithelial tissues such as the kidney, the type II isozyme catalyzes the glucocorticoid cortisol to the inactive metabolite cortisone, thus preventing illicit activation of the mineralocorticoid receptor. In tissues that do not express the mineralocorticoid receptor, such as the placenta and testis, it protects cells from the growth-inhibiting and/or pro-apoptotic effects of cortisol, particularly during embryonic development. Mutations in this gene cause the syndrome of apparent mineralocorticoid excess and hypertension. [provided by RefSeq, Feb 2010]
UniProt Code:	P80365
NCBI GenInfo Identifier:	30316367
NCBI Gene ID:	3291
NCBI Accession:	P80365.2
UniProt Secondary Accession:	P80365,Q13194, Q6P2G9, Q8N439, Q96QN8, Q9UC50, Q9UC51 Q9UCW5, Q9UCW6, Q9UCW7, A7LB28, C5HTY7,
UniProt Related Accession:	P80365
Molecular Weight:	44,127 Da
NCBI Full Name:	Corticosteroid 11-beta-dehydrogenase isozyme 2
NCBI Synonym Full Names:	hydroxysteroid 11-beta dehydrogenase 2
NCBI Official Symbol:	HSD11B2
NCBI Official Synonym Symbols:	AME; AME1; HSD2; HSD11K; SDR9C3
NCBI Protein Information:	corticosteroid 11-beta-dehydrogenase isozyme 2
UniProt Protein Name:	Corticosteroid 11-beta-dehydrogenase isozyme 2
UniProt Synonym Protein Names:	11-beta-hydroxysteroid dehydrogenase type 2; 11-DH2; 11-beta-HSD2; 11-beta-hydroxysteroid dehydrogenase type II; 11-HSD type II; 11-beta-HSD type II; NAD-dependent 11-beta-hydroxysteroid dehydrogenase; 11-beta-HSD; Short chain dehydrogenase/reductase family 9C member 3
Protein Family:	Corticosteroid 11-beta-dehydrogenase isozyme
UniProt Gene Name:	HSD11B2
UniProt Entry Name:	DHI2_HUMAN

	Western blot analysis of extracts of HepG2 cells, using HSD11B2 antibody (CAB8077) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 15s.
	Immunohistochemistry of paraffin-embedded human liver damage using HSD11B2 antibody (CAB8077) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded human stomach using HSD11B2 antibody (CAB8077) at dilution of 1:100 (40x lens).
	Immunohistochemistry of paraffin-embedded rat kidney using HSD11B2 antibody (CAB8077) at dilution of 1:100 (40x lens).