Cell Biology Antibodies 6
Anti-HOOK3 Antibody (CAB15536)
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- SKU:
- CAB15536
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Cell Biology
Description
| Antibody Name: | Anti-HOOK3 Antibody |
| Antibody SKU: | CAB15536 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 489-718 of human HOOK3 (NP_115786.1). |
| Application: | WB IF |
| Recommended Dilution: | WB 1:200 - 1:2000 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | LO2, HeLa, 293T, mouse heart, mouse kidney, rat lung |
| Immunogen: | Recombinant fusion protein containing a sequence corresponding to amino acids 489-718 of human HOOK3 (NP_115786.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | IALL QSLL DDAN LRKN ELET ENRL VNQR LLEV QSQV EELQ KSLQ DQGS KAED SVLL KKKL EEHL EKLH EANN ELQK KRAI IEDL EPRF NNSS LKIE ELQE ALRK KEEE MKQM EERY KKYL EKAK SVIR TLDP KQNQ GAAP EIQA LKNQ LQER DRLF HSLE KEYE KTKS QREM EEKY IVSA WYNM GMTL HKKA AEDR LAST GSGQ SFLA RQRQ ATSS RRSY PGHV QPAT AR |
| Gene ID: | 84376 |
| Uniprot: | Q86VS8 |
| Cellular Location: | Cytoplasm, Golgi apparatus, cytoskeleton |
| Calculated MW: | 83kDa |
| Observed MW: | 170kDa |
| Synonyms: | HOOK3, HK3 |
| Background: |
| UniProt Protein Function: | HOOK3: Probably serves as a target for the spiC protein from Salmonella typhimurium, which inactivates it, leading to a strong alteration in cellular trafficking. Component of the FTS/Hook/FHIP complex (FHF complex). The FHF complex may function to promote vesicle trafficking and/or fusion via the homotypic vesicular protein sorting complex (the HOPS complex). May regulate clearance of endocytosed receptors such as MSR1. Participates in defining the architecture and localization of the Golgi complex. Belongs to the hook family. |
| UniProt Protein Details: | Protein type:Adaptor/scaffold Chromosomal Location of Human Ortholog: 8p11.21 Cellular Component: centrosome; cis-Golgi network; cytoplasm; cytosol; Golgi apparatus; microtubule; microtubule organizing center; pericentriolar material Molecular Function:identical protein binding; microtubule binding; protein binding Biological Process: cytoplasmic microtubule organization and biogenesis; early endosome to late endosome transport; endosome organization and biogenesis; endosome to lysosome transport; Golgi localization; interkinetic nuclear migration; lysosome organization and biogenesis; negative regulation of neurogenesis; protein transport |
| NCBI Summary: | Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.[supplied by OMIM, Apr 2004] |
| UniProt Code: | Q86VS8 |
| NCBI GenInfo Identifier: | 14165274 |
| NCBI Gene ID: | 84376 |
| NCBI Accession: | NP_115786.1 |
| UniProt Secondary Accession: | Q86VS8,Q8NBH0, Q9BY13, D3DSY8, |
| UniProt Related Accession: | Q86VS8 |
| Molecular Weight: | 83,126 Da |
| NCBI Full Name: | protein Hook homolog 3 |
| NCBI Synonym Full Names: | hook microtubule tethering protein 3 |
| NCBI Official Symbol: | HOOK3 |
| NCBI Official Synonym Symbols: | HK3 |
| NCBI Protein Information: | protein Hook homolog 3 |
| UniProt Protein Name: | Protein Hook homolog 3 |
| Protein Family: | Protein Hook |
| UniProt Gene Name: | HOOK3 |
| UniProt Entry Name: | HOOK3_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HOOK3 antibody (CAB15536) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 60s. |
 | Immunofluorescence analysis of C6 cells using HOOK3 antibody (CAB15536) at dilution of 1:100. Blue: DAPI for nuclear staining. |
 | Immunofluorescence analysis of L929 cells using HOOK3 antibody (CAB15536) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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