Description
Antibody Name: | Anti-HOOK2 Antibody (CAB9337) |
Antibody SKU: | CAB9337 |
Antibody Size: | 50µL, 100µL |
Application: | Western blotting |
Reactivity: | Human, Mouse, Rat |
Host Species: | Rabbit |
Immunogen: | A synthesized peptide derived from human HOOK2. |
Application: | Western blotting |
Recommended Dilution: | WB 1:500 - 1:2000 |
Reactivity: | Human, Mouse, Rat |
Positive Samples: | HT-29, 293T, A-431, Mouse brain, Mouse heart, Rat brain |
Immunogen: | A synthesized peptide derived from human HOOK2. |
Purification Method: | Affinity purification |
Storage Buffer: | Store at -20°C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
Isotype: | IgG |
Sequence: | Email for sequence |
Cellular Location: | Cytoplasm, centrosome, cytoskeleton, microtubule organizing center |
Calculated MW: | 85kDa |
Observed MW: | 90KDa |
Synonyms: | HOOK2, HK2, protein Hook homolog 2 |
Background: | Hook proteins are cytosolic coiled-coil proteins that contain conserved N-terminal domains, which attach to microtubules, and more divergent C-terminal domains, which mediate binding to organelles. The Drosophila Hook protein is a component of the endocytic compartment.supplied by OMIM, Apr 2004 |
Western blot analysis of extracts of various cell lines, using at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s. |