Epigenetics & Nuclear Signaling Antibodies 2
Anti-HNRNPR Antibody (CAB5883)
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- SKU:
- CAB5883
- Product Type:
- Antibody
- Reactivity:
- Human
- Reactivity:
- Mouse
- Reactivity:
- Rat
- Host Species:
- Rabbit
- Isotype:
- IgG
- Antibody Type:
- Polyclonal Antibody
- Research Area:
- Epigenetics and Nuclear Signaling
Description
| Antibody Name: | Anti-HNRNPR Antibody |
| Antibody SKU: | CAB5883 |
| Antibody Size: | 20uL, 50uL, 100uL |
| Application: | WB IHC IF |
| Reactivity: | Human, Mouse, Rat |
| Host Species: | Rabbit |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human HNRNPR (NP_005817.1). |
| Application: | WB IHC IF |
| Recommended Dilution: | WB 1:500 - 1:2000 IHC 1:100 - 1:200 IF 1:50 - 1:200 |
| Reactivity: | Human, Mouse, Rat |
| Positive Samples: | SKOV3, LO2, HL-60, Mouse brain, Mouse spleen, Rat brain |
| Immunogen: | A synthetic peptide corresponding to a sequence within amino acids 500 to the C-terminus of human HNRNPR (NP_005817.1). |
| Purification Method: | Affinity purification |
| Storage Buffer: | Store at -20'C. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
| Isotype: | IgG |
| Sequence: | RGRG GGRG GRGA PPPP RGRG APPP RGRA GYSQ RGAP LGPP RGSR GGRG GPAQ QQRG RGSR GSRG NRGG NVGG KRKA DGYN QPDS KRRQ TNNQ QNWG SQPI AQQP LQQG GDYS GNYG YNND NQEF YQDT YGQQ WK |
| Gene ID: | 10236 |
| Uniprot: | O43390 |
| Cellular Location: | Cytoplasm, Nucleus, nucleoplasm |
| Calculated MW: | 59kDa/66kDa/70kDa/71kDa |
| Observed MW: | 71kDa |
| Synonyms: | HNRNPR, HNRPR, hnRNP-R |
| Background: | This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10. |
| UniProt Protein Function: | hnRNP R: Component of ribonucleosomes, which are complexes of at least 20 other different heterogenious nuclear ribonucleoproteins (hnRNP). hnRNP play an important role in processing of precursor mRNA in the nucleus. 2 isoforms of the human protein are produced by alternative splicing. |
| UniProt Protein Details: | Protein type:RNA splicing; RNA-binding; Spliceosome Chromosomal Location of Human Ortholog: 1p36.12 Cellular Component: nucleolus; nucleoplasm; ribonucleoprotein complex Molecular Function:protein binding Biological Process: gene expression; nuclear mRNA splicing, via spliceosome |
| NCBI Summary: | This gene encodes an RNA-binding protein that is a member of the spliceosome C complex, which functions in pre-mRNA processing and transport. The encoded protein also promotes transcription at the c-fos gene. Alternative splicing results in multiple transcript variants. There are pseudogenes for this gene on chromosomes 4, 11, and 10. [provided by RefSeq, Jul 2014] |
| UniProt Code: | O43390 |
| NCBI GenInfo Identifier: | 12230547 |
| NCBI Gene ID: | 10236 |
| NCBI Accession: | O43390.1 |
| UniProt Secondary Accession: | O43390,Q2L7G6, Q5TEH1, Q9BV64, S4R3J4, |
| UniProt Related Accession: | O43390 |
| Molecular Weight: | 59,953 Da |
| NCBI Full Name: | Heterogeneous nuclear ribonucleoprotein R |
| NCBI Synonym Full Names: | heterogeneous nuclear ribonucleoprotein R |
| NCBI Official Symbol: | HNRNPR |
| NCBI Official Synonym Symbols: | HNRPR; hnRNP-R |
| NCBI Protein Information: | heterogeneous nuclear ribonucleoprotein R |
| UniProt Protein Name: | Heterogeneous nuclear ribonucleoprotein R |
| Protein Family: | Heterogeneous nuclear ribonucleoprotein |
| UniProt Gene Name: | HNRNPR |
| UniProt Entry Name: | HNRPR_HUMAN |
 | Western blot analysis of extracts of various cell lines, using HNRNPR antibody (CAB5883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s. |
 | Immunohistochemistry of paraffin-embedded rat heart using HNRNPR antibody (CAB5883) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded human esophagus using HNRNPR antibody (CAB5883) at dilution of 1:100 (40x lens). |
 | Immunohistochemistry of paraffin-embedded mouse liver using HNRNPR antibody (CAB5883) at dilution of 1:100 (40x lens). |
 | Immunofluorescence analysis of U2OS cells using HNRNPR antibody (CAB5883) at dilution of 1:100. Blue: DAPI for nuclear staining. |
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